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Lecturer(s)
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Frébortová Jitka, doc. RNDr. Ph.D.
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Course content
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Introduction, cell types, basic classification and characteristics of microorganisms. Cell structure and function. Microscopic techniques. Growth and cultivation of bacteria. Classification and identification of bacteria. Metabolism of microorganisms: metabolism of chemoorganotrophs and chemolitotrophs, photosynthesis, anabolic pathways. Prokaryotic microorganisms, eukaryotic microorganisms: characteristic features. Fundamentals of virology. Essentials of molecular biology of prokaryots: DNA and RNA structure, replication, transcription, translation, protein folding and secretion, post-translational modifications, mechanisms of control of gene expression. Principles of bacterial genetics. Microbiology and biotechnology. Microorganisms and the environment, spreading of microorganisms, microorganisms in soil and water, interactions between microorganisms and plants, animals and humans. Applied microbiology: microbiology of food and beverages, microbiology of waste treatment. Antimicrobial medicines.
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Learning activities and teaching methods
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Lecture, Projection (static, dynamic)
- Preparation for the Exam
- 50 hours per semester
- Attendace
- 39 hours per semester
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Learning outcomes
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The goal of the course is to introduce fundamental principles of microbiology.
Student should be able to (after attending the course): - describe the structure and function of prokaryotic and eukaryotic microbial cell, methods of isolation and cultivation of microorganisms and methods of their growth control - define principles of bacterial classification and identification; characterize significant groups of prokaryotic as well as eukaryotic microorganisms; describe properties of viruses - explain principles of energy conservation by microbial cells and explain the differences between metabolic pathways; explain mechanisms of carbon fixation during autotrophic growth; explain mechanisms of nitrogen fixation - explain basic principles of bacterial molecular biology and genetics; describe principles of genetic engineering and give examples of application of microorganisms in biotechnology - describe the presence and importance of microorganisms in the environment; describe the impact of microorganisms on humans; give examples of application of microorganisms in food industry and wastewater treatment
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Prerequisites
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unspecified
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Assessment methods and criteria
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Mark, Written exam
Written examination (90 min), minimum 23 points out of 39 to pass. 39-36 points A; 35,75-32,75 points B; 32,5-29,5 points C; 29,25-26,25 points D; 26-23 points E; less than 23 points F.
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Recommended literature
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Madigan M. T., Martinko J. M., Dunlap P. V., Clark D. P. (2009). Brock Biology of Microorganisms, 12th edition. Pearson Benjamin Cummings.
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Madigan M.T., Martinko J.M., Dunlap P.V., Clark D.P. (2009). Brock Biology of Microorganisms, 12th Edition.
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