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Lecturer(s)
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Zatloukalová Martina, Mgr. Ph.D.
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Šatka Štefan, Mgr.
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Hanyk Jiří, Mgr.
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Novotná Renata, Ing. Ph.D.
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Bartek Josef, doc. MUDr. CSc.
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Kosina Pavel, Mgr. Ph.D.
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Ryšavá Alena, Mgr. Ph.D.
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Ulrichová Jitka, prof. RNDr. CSc.
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Vostálová Jitka, doc. RNDr. Ph.D.
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Dostál Zdeněk, Mgr. Ph.D.
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Rajnochová Svobodová Alena, doc. Ing. Ph.D.
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Dehner Jan, Mgr.
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Course content
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Lipid and lipoproteins metabolism, overview. Degradation and biosynthesis of saturated and polyunsaturated fatty acid. Metabolism of eicosanoids. Ketone bodies. Triacylglycerol, phospholipid and glycolipid metabolism. Cholesterol and bile acids metabolism. Metabolismus steroids and vitamin D. Insulin, glucagon, effect on carbohydrate and lipid metabolism. Pathobiochemistry of carbohydrates and lipids. Proteolysis. General pathways of amino acids catabolism. Amino acids biosynthesis, urea cycle. Metabolism of aromatic amino acids. Catecholamines. Tetrapyrroles biosynthesis and degradation. Bile pigments. Iron metabolism. Nucleotides-structure, function, metabolism. Nucleic acid, analysis. Gastrointestinal digestion and absorption. Detoxification function of the liver. Metabolism of xenobiotics. Glycosaminoglycans, proteoglycans. Glycoproteins. Biologically important radicals. Oxidative stress. Antioxidants. NO - Biosynthesis and function. Extracellular matrix, biosynthesis of extracellular proteins. Osteosynthesis. Biochemistry of muscle contraction: energy supply in skeletal and muscle heart. Biochemistry of neurodegenerative diseases. Biochemistry of vision. Minerals, trace elements: metabolic and biological role. Biochemical markers in clinical diagnosis. Cell models in biochemistry and toxicology. Information resources, work with literature and databases. The structure, properties, nomenclature of enzymes, factors affecting their activity. Determination of Michaelis constant of alkaline phosphatase in serum. Detection of enzymatic character of peroxidase reaction. Demonstration of substrate specificity: amylase and sucrase. Inhibition of enzyme activity of catalase by cyanide. Determination of optimum temperature of trypsin. Determination of plasma enzymes as diagnostic tools (lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase). Immunoanalytical techniques in determination of clinically important molecules. Acid-base balance. The metabolism of carbohydrates, lipids, proteins and tetrapyroles. Determination of the diagnostically important compounds (glucose, ketone bodies, glycated proteins, total, LDL- and HDL-cholesterol, triglycerides, bilirubin, hemoglobin, creatinine, urea). Analysis of urine, gastric juice, feces and bile duct stones. Microscopic examination of urinary sediment. Selected topics in biochemistry (student presentations).
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Learning activities and teaching methods
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Lecture, Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook), Demonstration, Training in job and motor Skils
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Learning outcomes
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The aim of the subject is to explain principles of biochemistry and the regulatory mechanisms of basic metabolic pathways. This knowledge is essential for interpretation of physiological and pathological events in human organism. Students are also trained in laboratory classes with res-pect to master simple biochemical analyses and the ability to interpret the results from laboratory tests.
The student who passes the subject will be equipped with knowledge on basic biochemical pathways and information on regulation of intermediary metabolism. Student will be able to interpret physiological and pathological events in human organism. In practice, student will be able to work according to good laboratory practice rules and to perform basic biochemical tests and to interpret laboratory results.
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Prerequisites
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Passing subject Medical chemistry.
LCH/VCA11 and KAR/VCB11 and BIO/VCB11 and CJA/VCB11 and LBF/VCB11 and NAN/VCB15 and PVL/VCB72 and HIE/VCB11
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Assessment methods and criteria
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Mark, Written exam
100 % attendance in practicals
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Recommended literature
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Devlin, T. M. (2010). Textbook of Biochemistry with Clinical Correlations. John Wiley & Sons.
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Dobrota, D. (2012). Lekárska biochémia. Martin: Osveta.
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Dvořáčková, S. a kol. (2007). Praktikum lékařské chemie a biochemie. Olomouc - UP.
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Champe, P. C., Harvey, R. A., Ferrier, D. R. (2007). Biochemistry. Lippincott Williams & Wilkins.
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Koolman, J., Röhm, K. H. (2012). Barevný atlas biochemie. Praha: Grada.
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Ledvina, M. a kol. (2009). Biochemie pro studující medicíny (1. a 2. díl). Praha - Karolinum, UK.
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Matouš, B. a kol. (2010). Základy lékařské chemie a biochemie. Praha: Galén.
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Murray, R. K. a kol. (2002). Harperova biochemie. Jinočany: H&H.
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Murray, R. K. a kol. (2012). Harperova ilustrovaná biochemie. Praha: Galén.
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Newsholme, E., Leech, A. (2010). Functional Biochemistry in Health and Disease. Wiley.
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Racek, J., Rajdl, D. (2021). Klinická biochemie. Praha, Galén.
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