| Course title | Optical Spectroscopies 1 |
|---|---|
| Course code | KEF/OSP1 |
| Organizational form of instruction | Lecture + Exercise |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 5 |
| Language of instruction | Czech |
| Status of course | Compulsory, Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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1. Introduction (spectroscopies - what is it and why do we use it, interaction of light and matter, energy of light, units, UV/VIS/NIR spectral range). 2. Sources and detectors of light (lasers, diodes, lamps, synchrotron rays, CW-, pulsed- and modulated-light, total internal reflection, principles of light detection, photomultipliers, photodiodes, CCD). 3. Absorption spectroscopy (derivation of Lambert-Beer law, presentation of spectra, photobleaching, transient absorption, experimental setup in absorption spectroscopy, experimental technic in absorption spectrocopy). 4. Absorption of biologically important molecules, perception of light, detection of light by living organisms. 5. Luminescence. Phenomenon of luminescence, Jabloński diagram, sorting of luminescences, sorting of photoluminescences, delayed fluorescence, elementary characteristics of luminescence. 6. Experimental technic for measurement of photoluminescence. Experimental setup in luminescence spectroscopy (comparison to absorption s.), spectral limits, sources, monochromators, samples, detectors, other optical elements. 7. Steady-state fluorescence (intensity, excitation-, emission and synchronnous spectra, quantum yield). 8.Fluorescence measurement using a pulsed light (time-domain) (kinetic, DAS, TRES). 9. Fluorescence measurement using a harmonically-modulated light (phase-domain). 10. Fluorophores. Intrinsic fluorophores, fluorescence probes, sensors, fluorescent proteins.10. Single-molecule spectroscopy. 11. Measurements using polarized light (anisotropy, rotational correlation time, Perrin equation, time-resolved measurement). 12. Fluorescence quenching (static, dynamic, Stern-Volmer equation). 13. FRET. 14. Fluorescence microscopy (confocal, FLIM, STED). 15. Measurement of intensity fluctuations (FIDA, FCS, FRAP). 16. Single-molecule spectroscopies. 17. Multiphoton excitation of fluorescence. 18. Solvent effects.
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| Learning activities and teaching methods |
| Lecture |
| Learning outcomes |
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<ul> <li> Sources of light and ways of excitation. Absorption spectroscopy. Experimental technique of absorption spectroscopy. Luminescence. Applications of fluorescent spectroscopy. Fluorophores.
Knowledge Define the main ideas and conceptions of the subject, describe the main approaches of the studied topics, demonstrate a theoretical knowledge for solution of model problems. |
| Prerequisites |
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unspecified
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| Assessment methods and criteria |
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Mark
<ul> <li> Knowledge within the scope of the course topics (examination) <li> Passing the oral examination </ul> |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Applied Physics (2019) | Category: Physics courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology - Specialization in Experimental Biology (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology - Specialization in Experimental Biology of Plants (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics - Specialization in General Biophysics (2024) | Category: Physics courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics - Specialization in Molecular Biophysics (2024) | Category: Physics courses | 2 | Recommended year of study:2, Recommended semester: Summer |