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Lecturer(s)
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Nožková Vladimíra, Mgr. Ph.D.
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Špundová Martina, doc. RNDr. Ph.D.
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Course content
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1. Parameters of water status (RWC, water potential and its components). Estimation of water content from reflectance spectra. Measurement of soil water status. Transpiration and water transport (gravimetry, potometry, gasometry, rate of xylem transport. Measurement of ion leakage through membranes. 2. Photosynthetically active radiation (PAR): sources, spectra, units, measurement. PAR in canopy, LAI. Optical phenomena in leaf (reflectance, transmittance). Analytical and non-destructive measurement of pigment content in plants. Chloroplast movement. Optical indexes used in plant physiology. 3. Plant description. Structure of photosynthetic apparatus of higher plants (leaf, chloroplast, thylakoid membranes, pigment-protein complexes). 4. Chlorophyll fluorescence, fluorescence induction (definition, properties, fluorimeters, measurement, parameters). Remote-sensing. Phenotyping. 5. Photosynthetic oxygen evolution. Rate of CO2 assimilation (IRGA, gasometric systems, parameters). Light- and CO2-curves of photosynthesis. 6. Plant senescence. Definition, importance, types of senescence. Senescence-induced changes. Methods in senescence studies. 7. Plant nanotechnology. Definition and properties of nanomaterials. Synthesis of nanoparticles. Methods of detection and characterization of nanoparticles. Nanotechnology in agriculture. Interaction of nanoparticles and plants. 8. Introduction to plant electrophysiology. Electrochemical potential. Nernst´s equation. Resting membrane potential, action and variation potential. Measurement of electric signals in plants: surface and intracellular methods, aphid-method, patch-clamp.
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Learning activities and teaching methods
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Lecture, Monologic Lecture(Interpretation, Training)
- Homework for Teaching
- 10 hours per semester
- Preparation for the Exam
- 40 hours per semester
- Attendace
- 28 hours per semester
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Learning outcomes
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Knowledge of principles and use of experimental methods in plant physiology.
Knowledge Define the main ideas and conceptions of the subject, describe the main approaches of the studied topics, recall the theoretical knowledge for solution of model problems.
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Prerequisites
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Knowledge in plant physiology. Basic knowledge of physics and chemistry. The subject LRR/FRP - Plant Physiology is recommended to pass.
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Assessment methods and criteria
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Mark, Oral exam
Passing the oral examination. Knowledge within the expent of the lecture.
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Recommended literature
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Boyer, J.S. (1995). Measuring Water Status of Plants and Soils. Academic Press, San Diego et al.
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Guo Y. (Ed.). (2018). Plant Senescence. Methods and Protocols.
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Kole C, Kumar DS, Khodakovskaya MV (Eds.). (2016). Plant Nanotechnology. Principles and Practices. Switzerland.
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Nobel, P.S. (1999). Physicochemical and Environmental Plant Physiology. Academic Press, San Diego et al.
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Reichardt K, Timm LC. (2020). Soil, Plant and Atmosphere. Concepts, Processes and Applications. Switzerland.
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Taiz, L., Zeiger, E. (2006). Plant Physiology. Sinauer Associates, Inc., Publisher, Sunderland, Massachussets.
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Volkov, A. G. (2006). Plant Electrophysiology. Theory and Methods.. Springer, Berlin, Heidelbnerg.
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von Caemmerer, C. (2000). Biochemical Models of Leaf Photosynthesis. CSIRO Publishing, Collingwood, Australia.
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Willey N. (2019). Environmental Plant Physiology. London and New York.
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