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Lecturer(s)
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Zalabák David, Mgr. Ph.D.
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Bergougnoux-fojtik Véronique Hélene, Ph.D.
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Course content
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The lecture covers the following topics: - Gene cloning tools and cloning strategies - Transfer of foreign genes into prokaryotic and eukaryotic cells - PCR technique and its uses - Mutagenesis - Creation and use of cDNA and genomic libraries. Gene chips, DNA microarrays, Affymetrix technology. - General characteristics of vectors for gene transfer to eukaryotes, selection markers. - Gene cloning in plants and its use. Gene transfer using Ti-plasmid derived vectors. - Gene silencing, gene knock-out, genome editing - Cloning of genes in animal cells, transgenic animals - Gene therapy, main strategies of gene therapy in vitro and in vivo. - Use of recombinant DNA methods in agriculture, industry and healthcare - Preparation of transgenic organisms. Risks of preparing transgenic organisms, safety rules for working with transgenic organisms. Ethical issues related to interspecies gene transfer and transgenic preparation organisms. GMO legislation. The content of the seminar is the discussion of cloning strategies and the independent development of a strategy for the functional expression of a given gene. Students will learn about software for molecular genetic analysis, sequence editing and comparison, primer design and restriction analysis. They will use and learn to navigate genetic databases on the Internet. The seminars will be validated by "homework", which will count towards the final exam.
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Learning activities and teaching methods
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Lecture, Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Preparation for the Course Credit
- 50 hours per semester
- Attendace
- 39 hours per semester
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Learning outcomes
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New trends in molecular biology is covered with application to medicine, biotechnology and agriculture.
Define main concepts, describe main approaches in genetic engineering
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Prerequisites
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Elementary knowledge of molecular biology.
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Assessment methods and criteria
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Mark, Written exam
In agreement with the subject. Writing exam.
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Recommended literature
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Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts K, Walter P. (2017). Molecular Biology of the Cell, 6th edition. Garland Science.
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Green M.R., Sambrook J. (2012). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
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Lodish H., Berk A., Zipursky S.L., Matsudaira P., Baltimore D., Darnell J. (2002). Molecular cell biology, 5th ed.. W.H. Freeman and Co., Basingstoke, England.
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Sabrook J., Fritsch E.F., Maniatis T. (2001). Molecular cloning. A laboratory manual. 3nd ed.. Cold Spring Harbor Laboratory Press.
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