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Lecturer(s)
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Navrátil Milan, prof. RNDr. CSc.
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Janovská Lucie, RNDr. Ph.D.
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Course content
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The structure and Complexity of virus genomes. Function and formation of virus particles: protein-protein and protein-nucleic acid interaction and genome packaging, virus receptors. Virus mutants. Genetic and non-genetic interactions between viruses. Virus genome replication and expresion strategies. Virus evolution and emerging viruses. Bacterial genome. Bacterial mutation and growth.Transformation, Conjugation, transduction. Evolutionary significance of parasexuality in bacteria. Saccharomyces cerevisiae model of cell biology and genetics: cell cycle, metabolism, sexuality, tetrad analysis. Molecular genetics of yeast: cell cycle regulation, signal pathways, regulation of transcription, homologous recombination. S. cerevisiae as a tool in molecular genetics, genomics and proteomics: genome, vectors, hybrid systems for interaction screening, in-vivo expression, DNA-microarrays. Unconventional and pathogenic yeast: heterologous expression in Hansenula polymorpha, pathogenic yeast - Candida and Cryptococcus, yeast dimorphism.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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Subject of the topic is the getting of knowledge about biological, molecular and genetic principles involved in life cycle of microorganisms.
Student is able to (after attending the course): - describe the molecular principles of virus structure. - describe the genetic and non-genetic interactions among viruses. - describe major types of replication strategies of RNA and DNA viruses. - describe GI of Bacteria, its structure and d function. - describe mechanism of DNA transfer among Bacterias. - describe the cell cycle at the cellular and genetic level. - discuss the application of viruses and yeasts in molecular genetics.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
oral exam in extent of the lectures
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Recommended literature
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Cann, A.J. (2001). Principles of Molecular Virology.. Elsevier Ltd.
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Griffiths, A. et al. (2005). Introduction to genetic analysis.. W.H. Freeman and Company, New York.
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Guthrie C., Fink G.R. (1991). Guide to Yeast Genetics and Molecular Biology, Parts A, B, and C.. Methods in Enzymology Vol. 194 (1991), 350 (2002), 351 (2002). Academic Press - Elsevier.
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Lewin, B. (2008). Genes IX. Sudbury, Mass: Jones and Bartlett.
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Navrátil M., Navrátilová L., Válová P. (2015). Kapitoly z molekulární biologie a genetiky mikroorganizmů. Vydavatelství UP, Olomouc.
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Rosypal, S. (1997). Úvod do molekulární biologie, I., II., III. a IV. díl. Brno.
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Snustad, D.P., Simmons, M.J. (2009). Genetika. Masarykova Univerzita, Brno.
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Snustad, D.P., Simmons, M.J. (2017). Genetika. Masarykova Univerzita, Brno.
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Šipický M., Šubík J. (1992). Genetika kvasiniek.. Veda, Vydavaťelstvo SAV, Bratislava.
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