Course: Practice of molecular-biological methods in diagnostics

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Course title Practice of molecular-biological methods in diagnostics
Course code KBC/MBM
Organizational form of instruction Exercise
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 7
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Zalabák David, Mgr. Ph.D.
  • Škrabišová Mária, Mgr. Ph.D.
Course content
unspecified

Learning activities and teaching methods
unspecified
Learning outcomes
Prerequisites
unspecified
KBC/MB and KBC/MBDM

Assessment methods and criteria
unspecified
Recommended literature
  • Cooper, P. C., Rezende, S. M. (2007). An overview of methods for detection of factor V Leiden and the prothrombin G20210A mutations. Int. Jnl. Lab. Hem., 29, 153?162.
  • Liang, F. T., Steere, A. C., Marques, A. R., Johnson, B. J., Miller, J. N., Philipp, M. T. Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi vlsE. J Clin Microbiol. 1999 Dec;37(12):3990-6.
  • Magnarelli, L. A., Anderson, J. F., Barbour, A. G. Enzyme-Linked Immunosorbent Assays for Lyme Disease: Reactivity of Subunits of Borrelia burgdorferi. The Journal of Infectious Diseases, Volume 159, Issue 1, January 1989, Pages 43?49.
  • Malekshahi, A., Khanizadeh, S., Fallahi, S. et al. (2022). Diagnostic power of one-step and two-step RT-qPCR methods to SARS?CoV?2 detection. BMC Infect Dis 22, 505.
  • Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., Hase, T. (2000). Loop-mediated isotherma amplification of DNA. Nucleic Acids Research, 28, 12, e63.
  • O'Malley, R. C., Barragan, C. C., Ecker, J. R. A user's guide to the Arabidopsis T-DNA insertion mutant collections. Methods Mol Biol. 2015;1284:323-42.
  • Różański, M., Walczak-Drzewiecka, A., Witaszewska, J. et al. (2022). RT-qPCR-based tests for SARS-CoV-2 detection in pooled saliva samples for massive population screening to monitor epidemics. Sci Rep 12, 8082.
  • Stepanova, A. N., Alonso, J. M. PCR-based screening for insertional mutants. Methods Mol Biol. 2006;323:163-72.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Analytical Biochemist (2024) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Summer