| Course title | Genetic Mapping and DNA Markers |
|---|---|
| Course code | KBC/GEMAP |
| Organizational form of instruction | Seminar |
| Level of course | Master |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 2 |
| Language of instruction | Czech |
| Status of course | Optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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1. Marker types definition. Markers based on phenotype, proteins and DNA sequence and comparison of their advantages and limitations. Types of polymorphism suitable for marker development. Markers based on hybridization and PCR. 2. Practical approaches for DNA marker development, identification of DNA sequences available for marker development. Use of protein sequence for marker identification and marker development. Utilization of bioinformatics and database tools. 3. Optimal approaches for marker development in target regions or whole genome "high-throughput" marker development approaches. Markers advantageous for map construction and phylogenetic analyses. 4. Basic principles for genome map construction. Types of maps and mapping populations and their utilization. Genotyping data analysis and map construction. Definitions of genetic and physical maps. 5. Available tools for construction of linkage maps. Demonstration using JoinMap and MultiPoint software. 6. Integration of genetic and physical maps. "Consensus" maps.
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| Learning activities and teaching methods |
Lecture
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| Learning outcomes |
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The course is designed for students of the Bioinformatics study program. In an adequate and concise form, students will learn practical ways of marker development from all possible DNA polymorphisms and marker utilization for genetic map construction and phylogenetic analysis.
Students will acquire practical knowledge about DNA marker development, their use and genetic map construction. |
| Prerequisites |
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Successful completion of bachelor's level study requirements; successful passing of subject KBB/GENOM.
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| Assessment methods and criteria |
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Oral exam
Successful passing of written colloquium - obtaining of at least 60% of credit points. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biochemistry (2022) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Bioinformatics (2021) | Category: Informatics courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Analytical Biochemist (2024) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Summer |