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Lecturer(s)
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Majeský Luboš, RNDr. Ph.D.
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Hroneš Michal, RNDr. Ph.D.
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Nádvorník Petr, RNDr. Ph.D.
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Kitner Miloslav, RNDr. Ph.D.
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Course content
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Information macromolecules (RNA, DNA, proteins, polysaccharides), basic techniques of collection and transport of samples from terrain to the laboratory. Extraction and storage of information macromolecules and their electrophoretic separation. Basic techniques of molecular biology, categories of molecular markers (codominant and dominant markers, izozymes, RFLP, RAPD, AFLP, microsatellites, SNPs, sequencing, NGS) a basic techniques of genetic variability analysis (genetic variability index, factors affecting genetic variability). Population genetic basics, measurement of population variability, Hardy-Weinberg equilibrium, dispersal forces disturbing equilibrium in populations (mutations, migration, selection, drift, inbreeding, assortative mating). Genetics and sex, sex determination types, genetic determination of sex, system with sex ratio deviated from 1:1, social insect, determination of sex in birds (CHD genes). Human evolution and migration, eugenic and dysgenic factors in his development, modern eugenic and dysgenic factors. Racial and ethnical differences.
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Learning activities and teaching methods
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Lecture, Monologic Lecture(Interpretation, Training)
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Learning outcomes
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Application of molecular biology techniques for the study of the ecological relationships in populations of prokaryotic and eukaryotic organisms.
Student is able to (after attending the course): - Explain the principles of molecular ecology methods and their application. - Propose conception and methodological approach for molecular ecology experiments.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam, Student performance
on oral exam in extent of the lectures
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Recommended literature
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& Kantartzi, S. K. (2013). Microsatellites: methods and protocols. New York, N.Y: Humana Press.
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Alberts, B., Wilson, J., & Hunt, T. (2015). Molecular biology of the cell. New York, N.Y: Garland Science.
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Freeland J. R. (2005). Molecular Ecology.
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Krebs, J. E., Goldstein, E. S., & Kilpatrick, S. T. (2014). Lewin's genes XI. Burlington, Mass: Jones & Bartlett Learning.
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Navrátil M., Navrátilová B., Válová P. (2015). Kapitoly z molekulární biologie a genetiky mikroorganismů. Olomouc.
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Wessner, D. R., Dupont, C., & Charles, T. (2013). Microbiology. Hoboken, N.J: Wiley.
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