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Lecturer(s)
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Doležel Jaroslav, prof. Ing. DrSc.
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Šimková Hana, Ing. CSc.
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Course content
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Genomics ? subject of study. Genome projects - organization and objectives. Genetic mapping in plants, animals and human. Mapping populations. Molecular markers ? types, characteristics, high-throughput platforms. Association mapping. Large-insert DNA libraries ? types, construction. Physical mapping ? cytogenetic physical maps, molecular physical maps. Automated procedure for physical mapping. Integration of physical and genetic maps. Positional cloning of genes. Comparative genomics. Whole-genome sequencing strategies (shotgun, clone-by-clone, reduced-representation strategies). The process of whole-genome sequencing, new-generation sequencing technologies. Annotation of DNA sequences. Single nucleotide polymorphisms (SNPs), discovery of SNPs and their use in genotyping. Gene expression and the transcriptome. DNA microarrays, their preparation and utilization. Determination of gene function. Approaches to functional genomics ? saturation forward genetics, high-throughput reverse genetics, fine-structure genetics. Genome projects in prokaryots, plants, animals and human.
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Learning activities and teaching methods
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Lecture, Monologic Lecture(Interpretation, Training)
- Homework for Teaching
- 20 hours per semester
- Preparation for the Exam
- 25 hours per semester
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Learning outcomes
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Study of genome organization and gene functions, and the modern methods and aproaches used for.
Student should be able to (after attending the course): - Explain the aids of Genomic projects. - Explain the construction of genetic and physical maps, as well as their use in gene cloning. - List possible procedures of whole-genome sequencing, including use of novel sequencing methods. - Describe the methods of detection and analysis of SNPs. - Describe the methods of analysis of whole-genome gene expression (DNA MicroArrays). - Define main procedures in Functional Genomics. - Explain the construction of the DNA BAC library and its screening.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam, Student performance
oral exam, in extent of the lectures
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Recommended literature
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Cullis, C.A. Plant Genomics and Proteomics. John Wiley and Sons. Inc., New York, 2004.
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Gibson, G., Muse, V.S. A Primer of Genome Science.. Sinauer Associates, Inc., Sunderland, 2004.
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Meksem, K., Kahl, G. The Handbook of Plant Genome Mapping. Weinheim, 2005.
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