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Lecturer(s)
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Fellner Martin, prof. RNDr. Ph.D.
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Ohnoutková Ludmila, Ing. Ph.D.
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Course content
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Laboratory practice will be focused on the methods of molecular and cell biology and their applications. 1) Use of recombinant DNA technology. 2) Preparation of vectors, cloning, methods of plant transformation using biolistic techniques, Agrobacterium and methods in planta. 3) Isolation of genomic and plasmid DNA, DNA quantification and quality analysis. 4) PCR detection of the transgenes and their localization 5) Microsatellites and their use for genotype identification.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration
- Attendace
- 24 hours per semester
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Learning outcomes
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Molecular biology is the scientific discipline that deals with the study of biological processes and their mutual functional relations at the molecular level. The principles and techniques are applied in various disciplines, such as genetic engineering.
After passing the course, the student should be able to: - Describe principles of molecular techniques and ways of their applications. - Name basic procedures and methods, including applications. - Characterize genetically modified organisms. - Prepare plant tissues and cells for genetic manipulations.
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Prerequisites
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Student should have basic knowledge of molecular biology
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Assessment methods and criteria
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Student performance
Attendance at exercises 100%; mastering the issue.
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Recommended literature
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Brown T.A. (2007). Klonování genů a analýza DNA. V Olomouci 2007.
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Buchanan B.B., Gruissem W., Jones R.L. (2001). Biochemistry and Molecular Biology of Plants.. Soc. Plant Physiol. Rockville, Maryland.
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Jackson F., Linskens H.F. (2000). Genetic Transformation of Plants (Molecular Methods of Plant Analysis).. Springer, 2003.
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Ondřej M., Drobník J. (2002). Transgenoze rostlin.. Akademia.
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Reece J. (2004). Analysis of Genes and Genomes.. West Sussex, England.
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