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Lecturer(s)
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Zatloukalová Martina, Mgr. Ph.D.
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Walterová Daniela, doc. RNDr. CSc.
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Ulrichová Jitka, prof. RNDr. CSc.
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Vostálová Jitka, doc. RNDr. Ph.D.
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Kosina Pavel, Mgr. Ph.D.
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Rajnochová Svobodová Alena, doc. Ing. Ph.D.
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Franková Jana, doc. Mgr. Ph.D.
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Dostál Zdeněk, Mgr. Ph.D.
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Course content
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Biochemistry, introduction. Proteins, structure, function. Supramolecular assemblies. Heteroproteins. Structure and function of hemoglobin. Immunoglobulins. Enzymes-structure, classification, mechanism of action. Cofactors and vitamins. Enzyme activity and its regulation. Enzymes in diagnosis. Signal transduction. Mechanism of hormone action. Biochemical function of subcellular organelles and membrane systems. Introduction to metabolic pathways. Bioenergetics. High-energy compounds. Biological oxidation. Respiratory chain, oxidative phosphorylation. Metabolism-overwiev. Citric acid cycle. AcetylCoA-central role in metabolism. Carbohydrate metabolism: overview. Glycolysis. Gluconeogenesis, pentose phosphate pathway, glycogen metabolism. Biomacromolecules in medicine Rules of study and directions for laboratory work. Information sources, databases. Laboratory ware. Laboratory techniques. Training of pippeting, weighting, measurement of volume, titration. Manganometric determination of H2O2 in medicinal solution. Determination of water hardness. Preparation of solutions, measurement of pH. Determination of H2SO4 in solution. Preparation, measurement and calculations of pH and buffers capacity. Chemical reactions of amino acids and proteins. Determination of isoelectric points of histidine. Chemical reactions saccharides. Polarimetric observations of D-glucose mutarotation. Chemical reactions of lipids. Determination of the iodine number of lipids. Revision, physical and chemical properties of biologically important compounds. Principles of chromatography. Thin layer chromatography of amino acids. Preparative chromatography of azo dyes on a thin layer. Identification of levomepromazine and its metabolites in biological materials by thin layer chromatography. Ion exchange chromatography - deionization of calcium chloride solution. Gel chromatography of hemolyzed blood. Isolation of plant alkaloids. Principles of electrophoresis. Isolation of albumins and globulins by fraction salting out. Electrophoretic separation of proteins. Separation of acidic, neutral and basic amino acids by electrophoresis. Principles of spectrophotometry. Quantitative determination of salicylates by photometry. Determination of proteins by spectrophotometry (Biuret method). Determination of vitamin C in fruit juices. Determination of nitrates. Practical photometric test. Revision, selected instrumental techniques, chromatography, electrophoresis, spectrophotometry.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook), Demonstration
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Learning outcomes
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The aim of the subject is to explain teoretical principles of biochemistry and the regulatory mechanisms of basic metabolic pathways. This knowledge is essential for interpretation of physiological and pathological events in human organism. Students are also trained in laboratory classes with respect to master simple biochemical analyses and the ability to interpret the results from laboratory tests.
The student who passes the subject will be equipped with theoretical knowledge on basic biochemical pathways and information on regulation of intermediary metabolism. Student will be able to interpret physiological and pathological events in human organism. In practice, student will be able to work according to good laboratory practice rules and to perform basic biochemical tests and to interpret laboratory results.
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Prerequisites
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Passing subject Medical chemistry.
LCH/VAA11 and BIO/VAA11
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Assessment methods and criteria
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Written exam, Didactic Test
100 % attendance in practicals
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Recommended literature
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Devlin, T. M. (2010). Textbook of Biochemistry with Clinical Correlations. John Wiley & Sons.
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Dvořáčková, S. a kol. (2007). Biochemistry: laboratory classes. Olomouc: Palacký University.
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Champe, P. C., Harvey, R. A., Ferrier, D. R. (2007). Biochemistry. Lippincott Williams & Wilkins.
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Koolman, J., Roehm, K.H. (2013). Color Atlas of Biochemistry. Thieme.
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Murray, R.K., Bender, D.A., Botham, K.M., Kennelly, P.J., Rodwell, V.W., Weil, P.A. (2022). Harper's Illustrated Biochemistry. McGraw-Hill.
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Newsholme, E., Leech, A. (2010). Functional Biochemistry in Health and Disease. Wiley.
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