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Vyučující
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Zatloukalová Martina, Mgr. Ph.D.
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Walterová Daniela, doc. RNDr. CSc.
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Ulrichová Jitka, prof. RNDr. CSc.
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Vostálová Jitka, doc. RNDr. Ph.D.
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Kosina Pavel, Mgr. Ph.D.
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Rajnochová Svobodová Alena, doc. Ing. Ph.D.
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Franková Jana, doc. Mgr. Ph.D.
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Dostál Zdeněk, Mgr. Ph.D.
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Obsah předmětu
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Biochemistry, introduction. Proteins, structure, function. Supramolecular assemblies. Heteroproteins. Structure and function of hemoglobin. Immunoglobulins. Enzymes-structure, classification, mechanism of action. Cofactors and vitamins. Enzyme activity and its regulation. Enzymes in diagnosis. Signal transduction. Mechanism of hormone action. Biochemical function of subcellular organelles and membrane systems. Introduction to metabolic pathways. Bioenergetics. High-energy compounds. Biological oxidation. Respiratory chain, oxidative phosphorylation. Metabolism-overwiev. Citric acid cycle. AcetylCoA-central role in metabolism. Carbohydrate metabolism: overview. Glycolysis. Gluconeogenesis, pentose phosphate pathway, glycogen metabolism. Biomacromolecules in medicine Rules of study and directions for laboratory work. Information sources, databases. Laboratory ware. Laboratory techniques. Training of pippeting, weighting, measurement of volume, titration. Manganometric determination of H2O2 in medicinal solution. Determination of water hardness. Preparation of solutions, measurement of pH. Determination of H2SO4 in solution. Preparation, measurement and calculations of pH and buffers capacity. Chemical reactions of amino acids and proteins. Determination of isoelectric points of histidine. Chemical reactions saccharides. Polarimetric observations of D-glucose mutarotation. Chemical reactions of lipids. Determination of the iodine number of lipids. Revision, physical and chemical properties of biologically important compounds. Principles of chromatography. Thin layer chromatography of amino acids. Preparative chromatography of azo dyes on a thin layer. Identification of levomepromazine and its metabolites in biological materials by thin layer chromatography. Ion exchange chromatography - deionization of calcium chloride solution. Gel chromatography of hemolyzed blood. Isolation of plant alkaloids. Principles of electrophoresis. Isolation of albumins and globulins by fraction salting out. Electrophoretic separation of proteins. Separation of acidic, neutral and basic amino acids by electrophoresis. Principles of spectrophotometry. Quantitative determination of salicylates by photometry. Determination of proteins by spectrophotometry (Biuret method). Determination of vitamin C in fruit juices. Determination of nitrates. Practical photometric test. Revision, selected instrumental techniques, chromatography, electrophoresis, spectrophotometry.
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Studijní aktivity a metody výuky
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Monologická (výklad, přednáška, instruktáž), Dialogická (diskuze, rozhovor, brainstorming), Metody práce s textem (učebnicí, knihou), Demonstrace
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Výstupy z učení
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The student should be familiar with theoretical principles of biochemistry, metabolism and its regulation necessary for interpretation of physiological and pathological processes in the human organism. Practical skills gained by the students should enable them to properly follow laboratory procedures, perform simple biochemical analyses, and evaluate laboratory rest results.
The student who passes the subject will be equipped with theoretical knowledge on basic biochemical pathways and information on regulation of intermediary metabolism. Student will be able to interpret physiological and pathological events in human organism. In practice, student will be able to work according to good laboratory practice rules and to perform basic biochemical tests and to interpret laboratory results.
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Předpoklady
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Passing subject Medical chemistry.
LCH/VAA11 a zároveň BIO/VAA11
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Hodnoticí metody a kritéria
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Písemná zkouška, Didaktický test
100 % attendance in practicals
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Doporučená literatura
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Devlin, T. M. (2010). Textbook of Biochemistry with Clinical Correlations. John Wiley & Sons.
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Dvořáčková, S. a kol. (2007). Biochemistry: laboratory classes. Olomouc: Palacký University.
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Champe, P. C., Harvey, R. A., Ferrier, D. R. (2007). Biochemistry. Lippincott Williams & Wilkins.
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Koolman, J., Roehm, K.H. (2013). Color Atlas of Biochemistry. Thieme.
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Murray, R.K., Bender, D.A., Botham, K.M., Kennelly, P.J., Rodwell, V.W., Weil, P.A. (2022). Harper's Illustrated Biochemistry. McGraw-Hill.
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Newsholme, E., Leech, A. (2010). Functional Biochemistry in Health and Disease. Wiley.
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