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Lecturer(s)
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Berka Karel, prof. RNDr. Ph.D.
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Hof Martin, prof. Dr. Ph.D.
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Ranc Václav, doc. RNDr. Ph.D.
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Course content
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First, key terminology and relations are mentioned. Then, specific spectroscopic methods are addressed, i.e., UV, IR, Raman, NMR, ESR, and fluorescence. Special attention is paid to spectral interpretation and the application of specific spectroscopic methods in practice. The students are also informed about the latest scientific knowledge with every spectroscopic method. Content of the presentations: 1. Spectrum-concept, fundamental characteristics and relations. Absorption spectra-introduction. 2. UV/VIS spectroscopy. 3. Absorption spectra and structure of substances. 4. IR spectroscopy. Introduction, basic vibrations. Instrumentation. 5. IR spectra-structure of substances. 6. Raman spectroscopy-its use for the study of the structures of biological macromolecules. 7. Resonance Raman spectroscopy. 8. Mössbauer spectroscopy. Introduction to NMR and ESR. 9. NMR spectra-instrumentation and applications. ESR spectra. 10. Emission spectra. Fluorescence spectroscopy-instrumentation. 11. Steady-state and time-differentiated fluorescence. Examples of applications for studying the structure of substances and their interactions. 12. Fluorescence correlation spectroscopy (FCS), relaxation of solvate layers. 13. Chirooptical methods /ORD, CD/. The study of the structure of biological macromolecules.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training)
- Preparation for the Exam
- 90 hours per semester
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Learning outcomes
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This course focuses on fundamental principles of spectroscopic methods.
ability to learn information, explain principles, interpret problems in spectral methods
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
Students sitting the examinations should be able to discuss two chosen questions.
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Recommended literature
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Donald Pavia, Gary Lampman, George Kriz, James Vyvyan. Introduction to Spectroscopy. 2014. 2021.
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John M Brown. Molecular Spectroscopy. 1998.
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