Course: Spectroscopic Methods

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Course title Spectroscopic Methods
Course code KFC/SM
Organizational form of instruction Lecture
Level of course Bachelor
Year of study not specified
Semester Winter
Number of ECTS credits 2
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Bužková Aneta, Mgr.
  • Berka Karel, prof. RNDr. Ph.D.
  • Prucek Robert, doc. RNDr. Ph.D.
Course content
1. Introduction, basic concepts, basic principles, classification. 2. UV-Vis absorption spectroscopy. Fundamentals of instrumentation, basic principles. Radiation sources. 3. Infrared spectroscopy. FTIR spectroscopy. Fundamentals of instrumentation, basic principles, vibrational-rotational spectra of substances and their interpretation. Examples of applications. 4. Raman spectroscopy - fundamentals, instrumentation, surface-enhanced Raman scattering (SERS) spectroscopy. Fundamentals and examples of possible uses. 5. Fluorescence, phosphorescence, luminescence. Fundamentals. Possible uses. 6. Atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), fundamentals, possible uses. ICP spectroscopy. 7. X-ray powder diffraction, electron and neutron diffraction. Generation and detection of X-ray radiation. Basic principles (Bragg's law), particle size measurement using low-angle X-ray scattering (SAXS). 8. Methods based on the emission/absorption of electrons/X-rays induced by the action of photons or particles. X-ray fluorescence spectroscopy (XRF). Basic principles (Moseley's law, Rayleigh and Compton scattering, secondary fluorescence). 9. Photoelectron spectroscopy (UPS, XPS/ESCA), Auger electron spectroscopy, X-ray absorption spectroscopy (XAS - EXAFS, XANES). 10. Mass spectrometry, interpretation of mass spectra. 11. Mössbauer spectroscopy. Mössbauer effect, experimental observation of the Mössbauer effect, hyperfine interactions, interpretation of Mössbauer spectra, low-temperature Mössbauer spectroscopy and spectroscopy in an external magnetic field. 12. Nuclear magnetic resonance. Nuclear magnetic moment. Magnetic moment in a magnetic field. Free precession. Spin and stimulated echo. NMR spectra. Chemical shift, signal splitting, double resonance, NMR of nuclei other than protons, NMR tomography. Magnetic resonance imaging (MRI). EPR spectroscopy.

Learning activities and teaching methods
Monologic Lecture(Interpretation, Training)
Learning outcomes
Students will be introduced to a variety of analytical methods, such as: UV-Vis absorption spectroscopy. Infrared spectroscopy. FTIR spectroscopy. Raman spectroscopy - basics, instrumentation, surface-enhanced Raman scattering (SERS) spectroscopy. Fluorescence, phosphorescence, luminescence. Atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES). X-ray powder diffraction, electron and neutron diffraction. Methods based on the emission/absorption of electrons/X-rays induced by the action of photons or particles. X-ray fluorescence spectroscopy (XRF). Photoelectron spectroscopy (UPS, XPS/ESCA), Auger electron spectroscopy, X-ray absorption spectroscopy (XAS - EXAFS, XANES). Mass spectrometry, interpretation of mass spectra. Mössbauer spectroscopy. Nuclear magnetic resonance.

Prerequisites
unspecified

Assessment methods and criteria
Student performance

Students sitting the examinations should be able to discuss two chosen questions
Recommended literature
  • Dheeraj Kumar Singh, Manik Pradhan, Arnulf Materny. (2021). Modern Techniques of Spectroscopy.
  • John C. Lindon. (2010). Encyclopedia of Spectroscopy and Spectrometry.
  • Sam Zhang, Lin Li, Ashok Kumar. (2008). Materials Characterization Techniques.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Applied Chemistry (2025) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter