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Lecturer(s)
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Kopečný David, Mgr.
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Kvasnicová Marie, Mgr. Ph.D.
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Kryštof Vladimír, prof. RNDr. Ph.D.
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Trofimenková Dominika, Mgr.
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Course content
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Light microscopy. Probing chemical composition of cell. Cell separation and cell cultures. Membranes, their compostion and characteristics. Cell transport. Osmotic features of cell. Animal cell. Plant cell. Cytochemical localization of cell components. Isolation of cells and cell counting. Cell viability assays. Cytotoxicity.
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Learning activities and teaching methods
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Laboratory Work
- Attendace
- 24 hours per semester
- Homework for Teaching
- 12 hours per semester
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Learning outcomes
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The aim of the course is to introduce students to the basic laboratory techniques of cell biology, to familiarize them with the laboratory environment, to ensure safe handling of biological material, and to train them in the evaluation of basic observations.
The student masters basic laboratory techniques (pipetting, solution preparation, microscopy), understands basic laboratory safety rules, can observe cells under a light microscope and record results, appreciates the importance of laboratory documentation and teamwork.
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Prerequisites
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No specific prerequisites; general biology and chemistry knowledge at high school level is recommended.
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Assessment methods and criteria
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Written exam, Analysis of Activities ( Technical works), Systematic Observation of Student
Full participation at the practicals is required (maximum two absences may be tolerated for serious reasons and the experiments may be conducted in a different class as decided by the teacher); all experimental tasks must be completed, reports submited and approved for each task. The class is concluded with a final written test.
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Recommended literature
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Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (1998). (2000). Základy buněčné biologie. Espero Publishing, Ústí nad Labem.
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Habrová, V. (1990). Mikroskopická technika. SPN Praha.
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Paleček, J. (1996). Biologie buňky, I. Základy mikroskopické cytologie. UK, Vydavatelství Karolinum, Praha.
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Razin S. E. (1999). Plant microtechnique and microscopy. Oxford University Press, Inc.
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