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Lecturer(s)
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Course content
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1) Introduction, limitations of classical plant breeding. 2) Traditional and contemporary approaches in plant genotyping and phenotyping. 3) QTL mapping, linkage disequilibrium and association mapping, recombinant inbred lines, new mapping populations. 4) Marker-assisted selection, genome-assisted selection and examples. 5) Isolation and functional analysis of genes, in silico gene prediction, gene cloning based on cDNA sequencing, positional gene cloning. 6) In vivo mutagenesis techniques, tilling, transposons. Use of mutagenesis in plant breeding. 7) Targeted genome engineering of model plants and crops. Disruption, addition, substitution and precise editing of genes. Structural changes of chromosomes. Meganucleases. 8) Zinc finger proteins, zinc finger nucleases and examples of successful application. 9) TAL effectors, TAL effector nucleases and examples of successful application. Nanotechnology and genome engineering. 10) Targeted plant transformation. Recombinase systems: CRE / lox, FLP / FRT and R / RS. Site-specific integration of a transgene. Recombinase-mediated cassette exchange. Examples of successful application. 11) Methods to produce marker-free and marker-free targeted transgenic plants. Cisgenesis. Examples of successful application. 12) Application of bioinformatics tools in molecular plant breeding. During the seminar, students will present "case study" presentations related to the topic of actual lecture. Based on the "cases" presented, students are further actively discussing the topic of a lecture from a practical point of view.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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Student will acquire an overview of the current applications and perspectives of molecular approaches in plant breeding.
Student will become familiar with current molecular approaches in plant breeding and understands their perspectives.
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Prerequisites
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Completion of the course CRH / ZGR Fundamentals of plant genetics and CRH / ZGI Fundamentals of genetic engineering
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Assessment methods and criteria
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Written exam
The prerequisite for the course is a written examination (test 1 hour 12 questions) with a minimum of 15 points out of 24 possible points. Credit is awarded for an active speech at the seminar.
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Recommended literature
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Jain, S. M., & Brar, D. S. (2010). Molecular techniques in crop improvement. Dordrecht: Springer.
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Murphy, D. (2007). Plant Breeding and Biotechnology. Cambridge University Press.
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Xu, Y. (2010). Molecular Plant Breeding. International Maize and Wheat Improvement Center (CIMMYT), CABI International.
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