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Lecturer(s)
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Hlaváčková Kateřina, Mgr. Ph.D.
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Medelská Miroslava, Mgr. Ph.D.
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Šamajová Olga, Mgr. Dr.
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Takáč Tomáš, doc. Ing. Ph.D.
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Šamaj Jozef, prof. RNDr. DrSc.
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Course content
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This course is an innovative combination of theoretical lectures and student seminars ("journal club"), which are closely linked to practical experiments on pre-selected student mini-projects. First week: -opening lectures about GFP technology and microscopy -preparation of plant material -transient transformation of Nicotiana benthamiana -wholemount immunolocalization of microtubules Second week: -student seminar (journal club) -microscopic in vivo observation of actin, microtubules and endosomes in stable transformed Arabidopsis plants using GFP technology -image analysis of the results from microscopic observations -results evaluation -presentation of the results by the students
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work
- Attendace
- 36 hours per semester
- Homework for Teaching
- 20 hours per semester
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Learning outcomes
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Students will gain knowledge and skills in modern GFP technology including methods of plant transformation with recombinant GFP constructs and analyses of GFP expression in transgenic cells and organs using confocal laser scanning microscopy. Students will work in mini-teams (3-4) under the guidance of professional staff.
Students will acquire practical and theoretical knowledge and experiences in the field of molecular plant cell biology.
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Prerequisites
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Students should be enrolled in Master degree programs in biological or chemical disciplines at the Faculty of Sciences, Palacký University.
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Assessment methods and criteria
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Student performance, Seminar Work
Students should be involved in Master degree programs in biological or chemical sciences at the Faculty of Natural Sciences UP. Students will present their results (prepared in Power Point) at the end of this course. They also have to submit detailed reports (formally structured as a scientific publication) no later than two weeks after the end of the course. The course is closely connected to the lecture Molecular cell biology of plants
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Recommended literature
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Conn M.P. (2010). Techniques in Confocal Microscopy. San Diego, USA.
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Dunwell J.M., Wetten A.C. (2012). Transgenic plants, Methods in Molecular Biology 847. New York.
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