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Lecturer(s)
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Kašpárková Jana, prof. RNDr. Ph.D.
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Brabec Viktor, prof. RNDr. DrSc.
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Course content
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1. Introduction: Definition of molecular biophysics. Intermolecular forces. Thermodynamics of transitions in biological systems - a brief survey. 2. Structure and function of nucleic acids: Structure of monomeric components of nucleic acids. Primary and secondary structure of DNA. Stability of DNA. Circular DNA. 3. Unusual structures. Nucleases. 4. Structure and properties of the chromosome. 5. DNA replication. 6. "RNA world". Types of RNA and structure. Transcription and translation. 7. The catalytic activity of nucleic acids - ribozymes a deoxyribozymes. Molecular evolution and the "RNA world". Aptamers. 8. Application of electrochemistry for studies of nucleic acids structure. 9. Structure and function of proteins: Function of proteins. Composition of proteins, structure, and properties of amino acids, the configuration of the peptide bond. Primary, secondary, the tertiary structure of proteins, forces stabilizing protein structures, the quaternary structure of proteins. 10. DNA binding proteins, their secondary structures. Effect of temperature and pH on protein structure. Chaperons, chaperonins. 11. Structure and function of other biopolymers: Saccharides - the function of saccharides, types of saccharides. Monosaccharides - structure, conformation, optical activity. Polysaccharides - conformation of the glycosidic bond. Glycoproteins. Examples and functions of polysaccharides. 12. Lipids - composition, the relationship between structure and physical properties. Composite lipids, sterols. Biophysical properties of lipids of biological significance. Physical properties of oils and lipids.
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Learning activities and teaching methods
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Lecture
- Attendace
- 26 hours per semester
- Preparation for the Exam
- 80 hours per semester
- Homework for Teaching
- 10 hours per semester
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Learning outcomes
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Structure and function of nucleic acids, amino acids. Structure and function of other biomolecules. Molecular biophysics of cellular membranes.
Knowledge Define the main ideas and conceptions of the subject, describe the main approaches of the studied topics, recall the theoretical knowledge for solution of model problems.
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Prerequisites
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Basic knowledge of biochemistry.
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Assessment methods and criteria
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Mark
Knowledge within the scope of the course topics (examination).
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Recommended literature
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Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2000). Základy buněčné biologie. Espero Publishing, Ústí nad Labem.
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Bergethon, P.R. (1998). The Physical Basis of Biochemistry. The Foundations of Molecular Biophysics. Springer.
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Kodíček, M., Karpenko, V. (2000). Biofyzikální chemie. Academia Praha.
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Tuszynski, J.A., Kurzynski, M. (2003). Introduction to Molecular Biophysics. CRC Press, Washington, DC.
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