| Course title | Practicum of basic laboratory methods |
|---|---|
| Course code | KBF/PZLM |
| Organizational form of instruction | Exercise |
| Level of course | Bachelor |
| Year of study | 2 |
| Semester | Summer |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
|---|
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| Course content |
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1. Principles of work in the chemical laboratory, chemical utensils, basic laboratory techniques (weighing, measuring volume, pipetting) 2. Preparation of solutions of the exact composition 3. Centrifugation 4. Conductometry, pH 5. Absorption spectrophotometry 6. Isolation of genomic DNA 7. DNA analysis using agarose gel electrophoresis 8. Preparation of protein extracts and determination of protein concentration in the cell lysate 9. Turbidimetry
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| Learning activities and teaching methods |
| unspecified |
| Learning outcomes |
|
The aim of this course is to acquaint students with basic methods commonly used in biophysical and biochemical laboratories.
The practice focuses on acquiring basic skills in chemical laboratory. |
| Prerequisites |
|
unspecified
KFC/BZP1 and KCB/OBBC ----- or ----- KCB/OBBI |
| Assessment methods and criteria |
|
unspecified
Successful completion of short test during each practice, attendance, proper reports (protocols), successful completion of the final colloquium. The course is intended for 2nd year students of Biophysics or Molecular biophysics. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biophysics - Specialization in General Biophysics (2024) | Category: Physics courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics - Specialization in Molecular Biophysics (2024) | Category: Physics courses | 2 | Recommended year of study:2, Recommended semester: Summer |