|
Lecturer(s)
|
-
Kopečný David, Mgr. Ph.D.
|
|
Course content
|
1. Optical rotational dispersion and circular dichroism, CD spectra of proteins and nucleic acids, static and dynamic light scattering, intereferometry on biological layer. 2. Small angle X-ray scattering (SAXS), Guinier graph, Kratky graph, paired distance distribution function, neutron scattering. 3. X-ray crystallography of biopolymers, crystal systems, Bravais lattices, Bragg's law, Miller indices, structural factors, measurement of X-ray diffraction, calculation of electron density map, phase problem, methods of anomalous scattering and isomorphic replacement, resolution. 4. Cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM), methods of positive and negative contrast. 5. Small-scale thermophoresis (MST), surface plasmon resonance (SPR). 7. Calorimetry, isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC). 8. Fluorimetry, differential scanning fluorimetry (DSF), Förster resonance energy transfer (FRET), bimolecular fluorescence complementation (BiFC), membrane binding analysis (MeRA). 8. Voltammetric and polarographic analysis of macromolecules. 9. Yeast two-hybrid system (Y2H), equilibrium dialysis, co-immunoprecipitation, filter binding assay.
|
|
Learning activities and teaching methods
|
Lecture
- Preparation for the Exam
- 55 hours per semester
- Attendace
- 26 hours per semester
|
|
Learning outcomes
|
Students obtain information about methods focused on studying macromolecular structures (proteins and nucleic acids), their stability and further binding interactions such as protein / protein, protein / ligand, protein / nucleic acid.
Deeper insight into modern methods of biochemistry and structural biology obtained by attending the lecture KBC / SSIM focused on methods of studying macromolecule inerations supplemented with illustrative examples.
|
|
Prerequisites
|
The course is designed for master students of biochemistry and other students in chemical and biological fields.
|
|
Assessment methods and criteria
|
Oral exam
Passing the oral exam.
|
|
Recommended literature
|
-
Nordén, B., Rodger, A., Dafforn, T. (2010). Linear Dichroism and Circular Dichroism: A Textbook on Polarized-Light Spectroscopy. Cambridge.
-
Serdyuk, I. N., Nathan R., Zaccai, J. (2007). Methods in Molecular Biophysics : Structure, Dynamics, Function. Cambridge.
-
Waseda, Y., Matsubara, E., Shinoda, K. (2011). X-ray Diffraction Crystallography. Heidelberg.
|