|
Lecturer(s)
|
-
Šebela Marek, prof. Mgr. Dr.
|
|
Course content
|
Preparation of biological samples. Methods for homogenization and extraction. Separation using semipermeable membrane. Concentration of samples. Precipitation methods. Centrifugation methods. Preparative and analytical centrifugation. Types of centrifugation rotors. Gradient centrifugation. Chromatographic methods. Physical forces and effects in chromatography. Theoretical aspects of chromatography. Classification of chromatographic carriers. Medium-pressure chromatography, perfusion chromatography. Electrophoretic methods for separation and analysis. Blotting techniques. Mass spectrometry and their application in biochemistry. Analysis of proteins, saccharides, lipids and nucleic acids. PCR methods. Chemical synthesis of peptides and oligonucleotides. Immunization and isolation of antibodies. Immunological methods. ELISA. Immunohistochemistry. Flow cytometry. Absorption and fluorescence spectroscopy. Infrared spectroscopy. Raman spectroscopy. Mössbauer spectroscopy. Chiroptic methods. Resonance spectroscopic methods. Crystallography. Calorimetry. Study of fast reactions. Electrochemical methods, polarography, voltammetry, and their application in biochemistry. Scattering (light, neutrons). Study of interaction biomacromolecule-ligand.
|
|
Learning activities and teaching methods
|
Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook)
- Preparation for the Exam
- 50 hours per semester
|
|
Learning outcomes
|
Students will acquaint themselves in detail with separation methods and methods of instrumental analysis that are used in current biochemistry.
Compare particular experimental methods, evaluate the effectiveness of the biochemical techniques.
|
|
Prerequisites
|
It is necessary to have knowledge at the level of the course KBC/BIME Biochemical Methods.
|
|
Assessment methods and criteria
|
Oral exam
the candidate is expected to conduct scientific discussion in broader context of the whole discipline.
|
|
Recommended literature
|
-
Gault V. A., McClenaghan N. H. (2009). Understanding Bioanalytical Chemistry: Principles and Applications. Wiley-Blackwell.
-
Johnstone R. A. W., Rose M. E. (2010). Mass Spectrometry for Chemists and Biochemists. Cambridge University Press.
-
Pingoud A., Urbanke C., Hoggett J., Jeltsch A. (2002). Biochemical Methods: A Concise Guide for Students and Researchers.. Wiley-VCH.
-
Pingoud A., Urbanke C., Hoggett J., Keltech A. (2002). Biochemical methods: A concise guide for students and researchers. Weinheim Německo.
-
Sadasivam S., Manickam M. (2009). Biochemical Methods. New Age International Pvt Ltd Publishers.
|