| Course title | Fundamentals of Molecular Biology |
|---|---|
| Course code | KBC/MBIOZ |
| Organizational form of instruction | Lecture |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Winter |
| Number of ECTS credits | 2 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Information macromolecules, structure of DNA and RNA. Molecular structure of genes and chromosomes. Replication of DNA, comparison of the replication in prokaryotes and eukaryotes. Errors and repairs of DNA. Transcription. Principles of the control of gene expression in prokaryotes and eukaryotes. Regulatory sequences, activators and repressors. Processing of eukaryotic mRNA. Translation. Structure of ribosomes. Genetic code and its importance. Structure of tRNA. Activation of amino acids. Initiation of the translation in prokaryotes and eukaryotes. Elongation. Termination. Posttranslational modifications of proteins. Chaperones and chaperonines. Fundamental methods of molecular biology. Isolation of DNA and RNA. Electrophoretic analysis of nucleic acids. Restriction analysis of DNA, construction of restriction maps, genome mapping. Polymerase chain reaction (PCR) in vitro. DNA sequencing, sequencing of genomes. Northern and Southern hybridization. Cloning strategies, transfer of DNA to bacterial and eukaryotic cells. Preparation of recombinant proteins. Site-directed mutagenesis. Transgenic organisms.
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| Learning activities and teaching methods |
Lecture
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| Learning outcomes |
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Obtaining fundamental knowledge in the field of molecular biology.
Define main concepts, describe main approaches used in molecular and cell biology. |
| Prerequisites |
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The course is intended for bachelor students in Biochemistry and to other chemistry and biology students.
KBC/BCH ----- or ----- KBC/UBC ----- or ----- KBC/UBCH |
| Assessment methods and criteria |
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Written exam
Obtaining at least 20 out of 30 points in a written test (answers to 15 questions, duration 1.5 hour) is needed to pass the exam. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biotechnology and Genetic Engineering (2022) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Bioorganic Chemistry and Chemical Biology (2020) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Biochemistry (2022) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Bioinformatics (2021) | Category: Informatics courses | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Bioinorganic Chemistry (2021) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Biophysics - Specialization in Molecular Biophysics (2024) | Category: Physics courses | 3 | Recommended year of study:3, Recommended semester: Winter |