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Lecturer(s)
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Zalabák David, Mgr. Ph.D.
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Bergougnoux-fojtik Véronique Hélene, Ph.D.
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Hajný Jakub, Mgr. Ph.D.
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Lenobel René, Mgr. Ph.D.
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Kopečný David, Mgr. Ph.D.
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Tarkowski Petr, doc. RNDr. Ph.D.
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Course content
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Typically the program includes the following topics: Methods of determination of protein structure. Introduction to metabolomics. Introduction to proteome analysis. Glycomics and lipidomics. Biosensors. Introduction to confocal and electron microscopy. Fundamentals of cloning and genetic engineering. Principles and applications of PCR. Cell cultures and production of recombinant proteins. Genetic transformation of cereals. Genetically modified organisms.
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Learning activities and teaching methods
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Lecture
- Attendace
- 26 hours per semester
- Preparation for the Exam
- 55 hours per semester
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Learning outcomes
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Obtaining specific advanced knowledge on modern trends in biochemietsry and related disciplines. Lectures given by experts on selected topics.
Define main concepts, ideas and principles of biochemical methods, outline procedures and strategies of biochemical methods.
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Prerequisites
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The course is intended for master students in Biochemistry and other chemistry and biology students.
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Assessment methods and criteria
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Written exam
Obtaining at least 20 out of 30 points in a written test (answers to 15 questions, duration 1.5 hour) is needed to pass the exam.
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Recommended literature
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Boyer R. (2012). Biochemistry Laboratory. Upper Saddle River, New Jersey.
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Wilson and Walker's. (2018). Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press; 8th edition.
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