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Lecturer(s)
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Jiravová Jana, Mgr. Ph.D.
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Šafářová Dana, Mgr. Ph.D.
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Nádvorník Petr, RNDr. Ph.D.
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Grycová Aneta, Mgr. Ph.D.
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Course content
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1) Safety guidelines and hygiene in a molecular biology laboratory. Basic conventions, units, and terminology. 2) Isolation of genomic DNA from plant tissues, determination of concentration and purity (spectrophotometry, nucleic acid gel electrophoresis). 3) Avian sex determination - PCR amplification of genomic DNA and electrophoretic analysis. 4) Isolation of immunoglobulins from blood serum, determination of IgG heavy and light chain MW (SDS-PAGE protein electrophoresis). 5) Total RNA isolation from plant tissues using a spin-column kit, determination of concentration and purity (spectrophotometry, gel electrophoresis). 6) Transformation of competent bacteria with plasmid DNA, antibiotic resistance transfer, and blue-white screening of transformed colonies.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration, Laboratory Work
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Learning outcomes
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To acquire practical skills in core molecular biology techniques - namely the extraction and analysis of nucleic acids (DNA, RNA) and proteins, PCR, and bacterial transformation.
Upon successful completion of the course, the student will be able to: Apply acquired laboratory skills to practical research in molecular genetics. Perform fundamental techniques in molecular biology research. Write a structured experimental report and critically discuss the results.
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Prerequisites
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unspecified
KBB/BZP
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Assessment methods and criteria
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Student performance
100% attendance in practical classes. Submission and approval of all laboratory reports (protocols) meeting the required standard by the given deadlines.
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Recommended literature
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Dle dalšího doporučení vyučujícím..
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Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2000). Základy buněčné biologie. Espero Publishing.
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Gálová. (2018). Metódy a techniky génových manipulácií. Nitra.
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Maniatis, T. et al. (1982). Molecular cloning: a laboratory manual.
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Navrátil, M., Kubaláková, M., Kučerová, L. Sylabus cvičení z molekulární biologie doplněný návody.
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Raclavský, V. (1998). Úvod do základních metod molekulární genetiky. Skriptum LF UP v Olomouci.
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