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Lecturer(s)
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Vrzalová Aneta, Mgr. Ph.D.
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Dvořák Zdeněk, prof. RNDr. DrSc.
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Course content
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Introduction, historic aspects and milestones of cell study, overview of modern techniques, challenges and aims of current cell biology. Methodology in cellular and molecular toxicology. Cell cultures, mechanisms of cellular damage, evaluation of cell damage. Probes and labels. cDNA and RNA probes in cell study. Techniques - Northern blot, Ribonuclease Protection Assay, Electrophoretic Mobility Shift Assay, Gene Reporter Assays. OMICS: Transcriptomics, proteomics, genomics, metabolomics - complex approaches for studying cell functions. Pharmacogenetics - genome and drug effects. Study of gene expression - gene reporter assay, RT-PCR, electrophoretic methods. Influencing gene expression - methods of transfection, transduction and gene silencing.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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This subject will give to students the knowledge on modern trends in the study of cell functions at molecular level; the methods used in practice and basic research. Subject compiles approaches of pharmacology, immunology, chemistry etc.
Studentis able to (after attending the course): - theoretically master modern techniques of cell and molecular biology
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written exam
70% test in extent of the lectures
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Recommended literature
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Alberts a kol. (2002). Molecular Biology of the Cell. New York.
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Rang, H.P., Dale, M.M., Ritter, J.M. (2003). Pharmacology. 5th ed., Churchill Livingstone, Edinburgh.
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