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Lecturer(s)
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Nádvorník Petr, RNDr. Ph.D.
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Course content
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Protein structure and function. Nucleic acids structure and function: DNA, mRNA, tRNA, r RNA, ribozyme, small RNA molecules. The genetic code. Genes. Non-repetitive and repetitive DNA sequences; transposones. Gene expression: transcription, regulatory elements, regulation and transcription factors, RNA splicing, RNA editing, synthesis of proteins (translation), sorting of proteins. Chaperone. Mechanism of DNA replication. Telomerase. Topoisomerases. Mutation mechanisms. DNA repair mechanisms. DNA recombination. Genetics of immunoglobulins. DNA cloning. Genome mapping; genomic librery and cDNA librery. Gel elektrophoresis. Hybridisation of nucleic acids, blotting techniques. Polymerase chain reaction. DNA sequencing. Gene therapy.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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Information macromolecules and methods for their analyses. Prokaryotic and eukaryotic genomes, their molecular structure and organization. Replication and expression of genetic information, mechanisms of their regualtion. Mutations. Gene transmissions.
Student is able to (after attending the course): - describe structure and function of nucleic acids and proteins - describe the structure and function of prokaryotic and eukaryotic genome - explain mechanism of replication of genetic information and the main mechanisms of its regulation - explain mechanism of expresion of gene expresion and the main mechanisms of its regulation - describe and explain causalities of genetic variability of organisms - describe and explain molecular causalities of spontaneous and induced mutations and their reparation - describe principles and applications of techniques used in molecular biology.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
oral exam in extent of the lectures, 3 randomly selected questions
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Recommended literature
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Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2000). Základy buněčné biologie. Espero Publishing, Ústí nad Labem.
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Lewin, B. (2008). Genes IX. Sudbury, Mass: Jones and Bartlett.
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Rosypal, S., Pantůček, R., & Doškař, J. (2001). Terminologie molekulární biologie: české odborné termíny, jejich definice a anglické ekvivalenty. Brno: Stanislav Rosypal.
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Rosypal S. Úvod do molekulární biologie, I., II. A III. díl, Brno, 1997..
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Snustad, D.P., Simmons, M.J. (2009). Genetika. Masarykova Univerzita, Brno.
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