Palacký University Olomouc
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for academic year 2024/2025
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Course: Genomics

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Course title Genomics
Course code KBB/GENOM
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 4
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Šimková Hana, Ing. CSc.
Course content
Genomics - subject of study. Genome projects - organization and objectives. Genetic mapping in plants, animals and human. Mapping populations. Molecular markers - types, characteristics, high-throughput platforms. Association mapping. Large-insert DNA libraries - types, construction. Physical mapping - cytogenetic physical maps, molecular physical maps. Automated procedure for physical mapping. Integration of physical and genetic maps. Positional cloning of genes. Comparative genomics. Whole-genome sequencing strategies (shotgun, clone-by-clone, reduced-representation strategies). The process of whole-genome sequencing, new-generation sequencing technologies. Annotation of DNA sequences. Single nucleotide polymorphisms (SNPs), discovery of SNPs and their use in genotyping. Gene expression and the transcriptome. DNA microarrays, their preparation and utilization. Determination of gene function. Approaches to functional genomics - saturation forward genetics, high-throughput reverse genetics, fine-structure genetics. Genome projects in prokaryots, plants, animals and human.

Learning activities and teaching methods
Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
Learning outcomes
Study of genome organization and gene functions, and the modern methods and aproaches used for.
Student is able to (after attending the course): - Explain the aids of Genomic projects. - Explain the construction of genetic and physical maps, as well as their use in gene cloning. - List possible procedures of whole-genome sequencing, including use of novel sequencing methods. - Describe the methods of detection and analysis of SNPs. - Describe the methods of analysis of whole-genome gene expression (DNA MicroArrays). - Define main procedures in Functional Genomics. - Explain the construction of the DNA BAC library and its screening.
Prerequisites
unspecified

Assessment methods and criteria
Oral exam

oral exam, in extent of the lectures
Recommended literature
  • Birren a kol. (1997). Genome analysis - Mapping Genomes. Cold Spring Harbor Laboratory Press, New York.
  • Brdička, R. Lidský genom na rozhraní tisíciletí. Grada Publishing, Praha, 2001.
  • Cantor, C.R., Smith, C.L. Genomics: The Science and Technology Behind the Human Genome Project, John Wiley and Sons, New York, 1999..
  • Cullis, C.A. Plant Genomics and Proteomics. John Wiley and Sons, Inc., New York, 2004.
  • Gibson, G., Muse, V.S. (2004). A Primer of Genome Science. Sinauer Associates, Inc., Sunderland.
  • Hunt, S. P., Livesey, F. Functional Genomics: A Practical Approach. Oxford University Press, Oxford, 2000..
  • Meksem, K., Kahl, G. (2005). The Handbook of Plant Genome Mapping. Wiley-VCH Verlag, Weinheim.
  • Primrose, S.B. Principles of Genome Analysis: A guide to mapping and sequencing DNA from different organisms. Blackwell Science Inc., 1998..
  • Rashidi, H.H., Buehler, L.K. Bioinformatics Basics. CRC Press, Boca Raton, 2000..


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Palacký University Olomouc, date of update: 02.05.2025 23:58. Data created for academic year 2024/2025