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Lecturer(s)
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Vrzal Radim, doc. Ing. Ph.D.
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Course content
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1. Safety in laboratory 2. DNA isolation from buccal mucous membrane 3. Measurement of isolated DNA concentration 4. Allele-specific PCR 5. Results analysis 6. Examples of inheritance from pedigrees
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work
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Learning outcomes
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SNP variants analysis and heredity coming from pedigree
Student is able to (after attending the course): - Apply the laboratory skills in human genetics research. - Write properly research reports aimed at solving the given problems and discuss the results
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Prerequisites
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Basic knowledge of genetics
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Assessment methods and criteria
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Written exam, Student performance
100% attendance, protocol elaboration (acceptable quality) , test (60% correctly answered questions)
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Recommended literature
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Brdička, R. Lidský genom na rozhraní tisíciletí. Grada Publishing, Praha, 2001.
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Bryšová V. a kol. Základy klinické genetiky. MU, Brno, 1995.
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Kapras, J. et al. (1996). Kapitoly z lékařské biologie a genetiky. Praha, UK Karolinum.
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Mueller, F.M., Young, I.D. (2001). Emeryś Elements of Medical Genetics. Churchill Livingstone.
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Schaaf, Christian P. (2012). Human Genetics: From molecule to medicine. Lippincott Wiliams and Wilkins, Baltimore.
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Sršeň, Sršňová. Základy klinickej genetiky. Osveta, Martin.
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Thompson, J. and Thompson, M. (2001). Genetics in Medicine. W.B. Saunders Company.
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