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Lecturer(s)
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Hrubý Jiří, Mgr.
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Illés Peter, RNDr. Ph.D.
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Smutná Tamara, RNDr. Ph.D.
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Course content
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Fundamentals of light and fluorescence microscopy. Cell viability assay. Effects of low temperatures on cells and cryopreservation. Determination of suspension culture density. Plant and animal cells. Autofluorescence, fluorescent labeling of DNA-containing structures. Interphase nucleus. Self-organization processes, biomembrane. Erythrocyte plasma membrane. Osmotic phenomena in cells.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Observation, Laboratory Work
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Learning outcomes
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Working with a light and fluorescence microscope, introducing selected basic methods used in cell biology, and observing cellular structutres.
Upon completion of the course, the student will be able to: - work with light and fluorescence microscope - work in a laboratory environment - perform simple experiments with eukaryotic cells, and to analyze and interpret the obtained data - write a report on the obtained results and discuss the findings - describe general procedures in cell study
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Prerequisites
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unspecified
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Assessment methods and criteria
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Student performance, Written exam
Attendance at practical classes, preparation of lab reports, and a minimum of 70% of points from the final written test.
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Recommended literature
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Habrová V. (1990). Mikroskopická technika. Praha.
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Paleček J. (1996). Biologie buňky, I. Základy mikroskopické cytologie. Praha.
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Razin S. E. (1999). Plant microtechnique and microscopy. Oxford.
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