| Course title | Microscopy and Image Analysis for Biochemists |
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| Course code | BOT/MOA |
| Organizational form of instruction | Lecture |
| Level of course | Master |
| Year of study | 1 |
| Semester | Winter |
| Number of ECTS credits | 3 |
| Language of instruction | Czech |
| Status of course | Compulsory-optional, Optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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The subject focuses on the principles, history and modern applications of light microscopy (including fluorescence and confocal m.) and other imaging techniques (SEM, TEM, atomic force microscopy, electron tomography), on the basic principles of specimen preparation for various microscopic methods, including correlative microscopy, on editing of microphotographs for publication purposes, on the mathematical methods for digital data processing, image analysis (histograms editing, dimension measurement, counting objects, 3D models). 1. Introduction, history, view, overview and comparison of direct and indirect methods of microscopy 2. Light microscopy and its modifications (phase contrast , polarized light, darkfield , Nomarski DIC) lenses 3. Fluorescence microscopy - principle of fluorescence, fluorochromes , transmission and epifluorescence microscopes, deconvolution 4. The preparation of biological samples for fluorescence microscopy - histochemical and immunohistochemical localization, fusion proteins 5. Confocal microscopy - spinning disc CM, laser scanning CM, multiphoton CM 6. Electron microscopy - history, principles, vacuum 7. SEM - scanning electron microscopy 8. TEM - transmission electron microscopy 9. Scanning tunneling microscopy, atomic force microscopy 10. Other imaging methods and their combinations (correlation microscopy, near-field optical microscopy, magnetic resonance imaging) 11. Acquaintance with current trends in microscopy - invited speakers 12. Acquaintance with current trends in microscopy - short presentation of students on a selected topic
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| Learning activities and teaching methods |
| Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration, Projection (static, dynamic) |
| Learning outcomes |
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Students will get familiar with microscopic techniques and software for image processing.
After completing the course the student should: - apply microscopic techniques - process image data |
| Prerequisites |
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Simultaneous completion of the course BOT/MOAC
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| Assessment methods and criteria |
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Oral exam, Written exam
Participation + essay |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Bioinformatics (2021) | Category: Informatics courses | - | Recommended year of study:-, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Biotechnology and Genetic Engineering (2019) | Category: Chemistry courses | 1 | Recommended year of study:1, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Plant Pathology (2019) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Winter |