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Lecturer(s)
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Kitner Miloslav, RNDr. Ph.D.
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Majeský Luboš, RNDr. Ph.D.
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Course content
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The aim of this course is to acquire basic skills of selected methods of molecular biology, which are currently being used in plant breeding, espetially for plant genotyping: isozyme analysis, RFLP RFLP (restriction fragment lenght polymorphism), AFLP (amplified fragment lenght polymorphism), SSR (simple sequence repeats, microsatellites). The use of these methods for cultivar identification, seed homogeneity testing, hybrid detection, genotyping, MAS (marker-assisted selection). Students will acquire basic methods of DNA isolation, amplification, separation and detection of PCR products, work with databases and statistic software.
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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This course is pointed the basic theoretical and practical techniques of molecular biology, currently used in plant breeding: isozyme anylysis, RFLP restriction fragment lenght polymorphism), AFLP (amplified fragment lenght polymorphism), SSR (mikrosatelites). The use of this tehniques for cultivar identification, hybrid detection, genotyping, MAS (marker-assisted selection). Students will be able to perform DNA isolation, PCR and electrophoretic techniques, work with databases and statistical software.
- Genetic diversity - Introduction to genetics - Parental material and genetic variability production - Introduction to Genetic Engineering - Molecular markers
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
oral exam: three questions a) general characteristics of DNA structure and its biological functions, PCR and its modifications b) principles of molecular markers techniques c) overview of the use of molecular markers in plant breeding
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Recommended literature
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Chaloupek O. 2000. Genetická diverzita, šlechtění a semenářství. Academia, Praha, 311 pp..
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Mazura I., Michalová K., Brdička R., Mácha J. 2001. Speciální metody molekulární biologie. Univerzita Karlova v Praze, Karolinum, 101pp..
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Weising K., Nybom H., Wolff K., Kahl G. 2005. DNA fingerprinting in plants. Principles, methods and applications (2nd ed). CRC Press, Boca Raton, FL, USA, 444pp..
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