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Lecturer(s)
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Ondřej Vladan, doc. RNDr. Ph.D.
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Navrátilová Božena, RNDr. Ph.D.
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Šenkyřík Josef Baltazar, Ing.
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Král Daniel, Mgr.
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Course content
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Lectures: History of biotechnologies, division, green biotechnology. Potential of plant cell and principle of plant tissue culture (technigues preserving and technigues increasing genetic variability of plants) and application in practice. Using of plant biotechnologies in agriculture, pharmaceutical industry, keeping the gene pool and phytoremediation. GMO of field crops. Application biotechnological methods in pea,bean and flax breeding. The future of plant biotechnology. Practices: Preparation of media, establishment of aseptic culture. Micropropagation, androgenesis, embryogenesis. The basics of working with GMOs. Agrobacterium genetic transformation of peas and beans, and the effect of co-cultivation period on transformation efficiency. Evaluation and photodocumentation of experiments.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration, Laboratory Work
- Attendace
- 26 hours per semester
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Learning outcomes
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The aim is to build on the general subjects (general biology, plant physiology, genetics, ecology) two-field teaching students study "Biology Teacher for Secondary Schools" a single-subject study "Botany" and expand knowledge in the field of biotechnology, particularly plant thereby increasing not only an overview of modern biotechnology, but also their competitiveness in their application in practice after graduation. The aim of the course established for students is also a direct confrontation with practice. Students will be able to integrate their acquired knowledge into biology teaching at primary and secondary schools, in interest clubs, seminars, and in the laboratory.
Knowledge - basic terms in area of plant biotechnologies using of plant biotechnologies in practice The student will gain practical experience working in an aseptic environment with selected basic methods of explant cultures used in plant research and breeding - preparation of plant material, initiation of explant cultures, establishment of experiments within the methods - anther culture, embryo culture and plant micropropagation. During the semester, students work on and evaluate the established experiments together.
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Prerequisites
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Student is able to orientation in the issue of plant biotechnologies,methods, their practices and questions of genetic modified plants (benefits and disadvantages).
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Assessment methods and criteria
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Dialog
Participation in the exercises. Own a short presentation (5 -10 min) on the topic of biotechnology.
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Recommended literature
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Heldman D.R., Wheler M.B., Hoover D.G. Encyclopedia of Biotechnology in agriculture and food..
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Kreuzer H., Massay A. (2005). Biology and biotechnology.. Washington.
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Procházka S. a kol. (1998). Fyziologie rostlin..
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Ram, A. (2010). Biotechnology: theory and practice. Jaipur.
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Smith J.E. (ed.). Biotechnology, 3rd Edition, Cambridge University Press 1998, ISBN 0-521-44911-1.
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Trigiano R.N., Gray D.J. (2005). Plant development and biotechnology..
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