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Lecturer(s)
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Pauk Volodymyr, Mgr. Ph.D.
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Lemr Karel, prof. RNDr. Ph.D.
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Course content
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Introduction to mass spectrometry, terminology, and the origin of mass spectra. Determination of molecular mass and elemental composition, utilization of isotopic peaks, accurate mass. Nitrogen rule, double bond equivalent. The scheme of a mass spectrometer, resolution. Mechanisms of ionization, design of selected ion sources and their application (electron ionization - EI, chemical ionization - CI, atmospheric pressure chemical ionization - APCI, atmospheric pressure photoionization, electrospray ionization - ESI, desorption electrospray ionization - DESI, direct analysis in real time - DART, thermospray - TSP, fast atom (ion) bombardment - FAB, CF-FAB, FIB, field ionization - FI, field desorption - FD, laser desorption ionization - LDI, matrix-assisted laser desorption ionization - MALDI, other desorption techniques). Design of mass analyzers - principles and theory of ion separation in a gas phase (sector instrument, quadrupole, 3D and linear ion trap, ion cyclotron resonance, orbitrap, time of flight). Tandem mass spectrometry - instrumentation (MS/MS, MSn). Induced dissociation (CID, SID). Detection and registration of ions, vacuum systems. Mass spectrometry in structural analysis. Introduction to ion fragmentation after electron ionization, selected fragmentation processes, rearrangements.
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Learning activities and teaching methods
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Lecture
- Attendace
- 13 hours per semester
- Preparation for the Course Credit
- 17 hours per semester
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Learning outcomes
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To provide students with more detail knowledge of mass spectrometry.
Define main concepts, describe main approaches, present the basic interpretation of mass spectra
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Prerequisites
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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Assessment methods and criteria
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Oral exam
Student has to be able to discuss principles and influences of important parameters of a mass spectrometric measurement, to obtain basic information from mass spectra, and to suggest suitable solutions of an analysis by mass spectrometry in different fields of application.
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Recommended literature
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Dass Ch.: Fundamentals of contemporary mass spectrometry, John Wiley & Sons, Hoboken 2007..
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de Hoffmann E., Stroobant, V.: Mass Spectrometry. Principles and Applications. John Wiley and sons, Chichester, 2002..
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Gross J. H.: Mass Spectrometry, A Textbook, 3. vyd. Springer International Publishing AG, Cham 2017..
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Niessen W. M. A.: Liquid chromatography-mass spectrometry, 3. vyd. CRC Press, Taylor & Francis Group, Boca Raton 2006..
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Niessen W.M.A.: Liquid Chromatography-Mass Spectrometry, Marcel Dekker, New York, 1999..
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Scientific publications devoted to mass spectrometry (mass spectrometric and analytical journals)..
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Sparkman O. D., Penton Z. E., Kitson F. G.: Gas chromatography and mass spectrometry: a practical guide, 2. vyd. Academic Press, Burlington 2011.
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Technical and application notes of leading producers of mass spectrometers..
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