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Lecturer(s)
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Milde David, doc. Ing. Ph.D.
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Jirovský David, doc. RNDr. Ph.D.
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Course content
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Liquid chromatography - paper and thin-layer chromatography, column chromatography. Instrumentation - solvent delivery systems, injection devices, columns, detectors, auxiliary devices. Retention in HPLC, principles of separation (normal-phase and reversed-phase liquid chromatography, ion-pair chromatography, liquid-liquid chromatography, separation of chiral compounds, ion-exchange chromatography, size-exclusion and affinity chromatography). Hyphenation of separation and spectral techniques (GC/MS, GC/IR, HPLC/MS, HPLC/FTIR, HPLC/NMR, CE/MS).Electromigration methods (electrophoretic mobility, electroosmotic flow, capillary electrophoresis, isotachophoresis). Mass spectrometry- origin of mass spectra; a scheme of mass spectrometer, resolution. Selected ion sources (electron ionization - EI, chemical ionization - CI, atmospheric pressure chemical ionization - APCI, electrospray ionization - ESI, desorption electrospray ionization - DESI, direct analysis in real time - DART, fast atom (ion) bombardment - FAB, FIB, laser desorption ionization - LDI, matrix assisted laser desorption ionization - MALDI). Mass analyzers - sector instruments, quadrupole, ion trap, time of flight, ion cyclotron resonance, orbitrap. Tandem mass spectrometry. Detection and registration of ions, vacuum systems. Mass spectrometry in a structural analysis. Quantitative analysis. Mass spectrometry of inorganic compounds. Spectrometry. Electromagnetic radiation. Absorption, emission and luminescence of radiation. Fundamental parts of spectrometers: radiation sources, monochromators, detectors. Atomic spectrometry - atomic absorption and fluorescence spectrometry, optical emission spectrometry (flames, plasma and electrical discharges). X-ray fluorescence. Application of atomic spectrometry.Molecular spectrometry - UV/Vis absorption spectrometry (theory, instrumentation, application). Fluorescence and phosphorescence of molecules. IR spectrometry (molecular vibrations and rotations, instrumentation, application). Raman spectrometry. NMR: principle, NMR spectrometer, NMR spectrum, analytical applications. Methods without energy exchange between radiation and matter: refractometry, polarymetry, turbidimetry, nephelometry. Electroanalytical Methods Classification of Electrochemical Methods. Electrochemical Cells, Electrodes. Potentiometric Methods of Analysis - Instrumentation, Ion-Selective Electrodes, Measurement of pH. Potentiometric Titrations. Voltammetry - Instrumentation, Electrode Process, Current-Potential Curves. Basic Voltammetric Techniques - DC Polarography and Voltammetry, Normal and Differential Pulse Voltammetry, Electrochemical Stripping Analysis. Titrations with Polarized Electrodes. Coulometry and Electrogravimetry - Instrumentation, Controlled-Potential and Controlled-Current Techniques. Conductometric Methods of Analysis - Conductometric Titrations, Measurement of Specific Electric Inductive Capacity.
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Learning activities and teaching methods
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Lecture
- Attendace
- 52 hours per semester
- Homework for Teaching
- 39 hours per semester
- Preparation for the Course Credit
- 89 hours per semester
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Learning outcomes
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The course provides the introduction to the field of instrumental techniques used in analytical chemistry
Explain the principles of instrumental methods of chemical analysis, interpret a problem, describe the solution of analytical problems
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Prerequisites
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The knowledge of basics of general, organic, anorganic, physical and analytical chemistry is recommended.
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Assessment methods and criteria
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Mark, Written exam
written final test, it is necessary to achieve at least 50% of all the points.
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Recommended literature
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Atomová spektroskopie- T. Černohorský, P. Jandera, Univerzita Pardubice 1997.
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Barek J., Opekar F., Štulík K.: Elektroanalytická chemie (skriptum). Karolinum, Praha 2004.
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de Hoffmann E., Stroobant, V.: Mass Spectrometry. Principles and Applications. John Wiley and sons, Chichester, 2002..
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Foret F., Křivánková L., Boček P.: Capillary Electrophoresis (Radola B. J., ed.), VCH Verlagsgesellschaft Weinheim, 1993..
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Harris D. C.: Quantitative Chemical Analysis. W. H. Freeman and Company, New York 2003.
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Harvey D.: Modern Analytical Chemistry. McGraw-Hill, New York 2000.
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Heiger D. N.: High Performance Capillary Electrophoresis - An Introduction, Hewlett-Packard GmbH Waldbronn, 1992..
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Holčapek M. (editor): Spojení vysokoúčinné kapalinové chromatografie a hmotnostní spektrometrie, Spektroskopická společnost Jana Marca Marci a Univerzita Pardubice, Pardubice, 2001..
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Churáček J. a kol.: Analytická separace látek, SNTL Praha, 1990..
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I. Wilson, C. Poole, M. Cooke: Encyclopedia of separation science. Academic Press, San Diego, London, Boston, New York, Sydney, Tokyo, Toronto 2000..
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Jandera P.: Molekulová spektroskopie v organické analýze. Univerzita Pardubice, Pardubice, 1999..
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Kellner R., Mermet J.-M., Otto M., Valcárcel M., Widmer, H. M.(eds.): Analytical Chemistry. A Modern Approach to Analytical Science. Wiley-VCH, Weinheim 2004.
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Li S. F. Y.: Capillary Electrophoresis, Journal of Chromatography Library 52, Elsevier Amsterodam, 1993..
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Modern Analytical Chemistry, D. Harvey, The Mc Graw Hill 2000.
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Modern Practice of Gas Chromatography Ed. R.L. Grob, E.F. Barry, Wiley-Interssience , 2004.
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Niessen W.M.A.: Liquid Chromatography-Mass Spectrometry, Marcel Dekker, New York, 1999..
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Opekar F. a kol.: Základní analytická chemie (skriptum). Karolinum, Praha 2003..
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Poole C., F., Poole S., K.: Chromatography Today, Elsevier, Amsterdam, 1991..
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Principles of Instrumental Analysis, D.A.Skoog, F.J. Holler, T.A. Nieman , Brooks/cole Thomson Learning 1997.
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R. E. Ardrey: Liquid Chromatography - Mass Spectrometry: An Introduction. John Wiley & Sons, Chichester 2003..
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Štulík K. a kol: Analytické separační metody, Karolinum UK Praha 2004.
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Ubik K.: Fyzikálně-chemické metody, část 2, Hmotnostní spektrometrie, Ústav organické chemie a biochemie AV ČR, Praha, 2000..
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Vřešťál J. (editor): Hmotnostní spektrometrie, Masarykova Univerzita, Brno, 1998.
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