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Lecturer(s)
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Petr Jan, doc. RNDr. Ph.D.
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Course content
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1. Introduction, principles of working with biological material, extraction of cellular content, centrifugation. 2. Methods of studying proteins, protein sequencing, study of post-translation modifications, crystallization, and structure of proteins. 3. Proteomics and proteomic approaches. 4. Affinity chromatography and electrophoresis, study of interactions. 5. Enzymes in analytical chemistry, coupled reactions, biosensors. 6. Analysis of nucleic acids, PCR, sequencing, genomics, aptamers. 7. Immunochemistry, antibodies, immunogenicity, haptens, immunochemical methods, immunoelectrophoresis, ELISA. 8. Microscopy and fluorescence microscopy, flow cytometry. 9. Micromanipulation, analysis of single cells. 10. Metabolomics. 11. Imaging. 12. Molecular biology methods, CRISPR-Cas9.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 26 hours per semester
- Preparation for the Exam
- 54 hours per semester
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Learning outcomes
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Theoretical as well as practical aspects of bioanalytical methods (methods that use biospecific interactions between molecules, or methods used in the biochemical laboratories) are introduced to students.
Students will be able to describe basic theoretical principles as well as practical aspects of bioanalytical techniques.
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Prerequisites
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The knowledge of basics of analytical chemistry is highly recommended.
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Assessment methods and criteria
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Written exam
The examination is done by a written test; minimally 60 % of points should be obtained for passing.
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Recommended literature
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Cibíček a kol. (2014). Principy a využití vybraných analytických metod v laboratorní medicíně. Vydavatelství UP Olomouc.
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Chromý V. a kol. (2011). Bioanalytika: Analytické metody v klinické chemii a laboratorní medicíně. Masarykova univerzita, Brno.
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Králová B. a kol. (2014). Bioanalytické metody. VŠCHT Praha.
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Lottspeich, F.; Engels, J.W. (Eds.). (2018). Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology. Wiley-VCH, Weinheim.
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Manz A. a kol. (2015). Bioanalytical Chemistry. Imperial College Press.
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Matysik F.-M. (2014). Advances in Chemical Bioanalysis. Springer.
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