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Lecturer(s)
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Lemr Karel, prof. RNDr. Ph.D.
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Course content
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Introduction, an application of mass spectrometry in organic analysis, history, and development. Structural analysis of small organic molecules (structure verification, e.g., identification of impurities in pharmaceutical substances, identification of metabolites), determination of molecular mass and elemental composition, fragmentation after electron ionization, collision-induced dissociation, fragmentation pathways, and the study of fragmentation. Application of ESI-MS, optimization of the ion source. Coupling of mass spectrometry and separation techniques. GC/MS in a toxicological analysis (databases of spectra). HPLC/MS - optimization of analytical methods, limits of used interfaces, and their comparison (e.g., ESI vs. APCI). Applications of HPLC/MS, matrix effects. Examples of mass spectrometry applications: peptides and their fragmentation (sequence of amino acids), analysis of metabolites, biomacromolecules, toxicological analysis, etc. Advances in mass spectrometry. Ion mobility-mass spectrometry (theory, instrumentation, examples of applications). Discussion of the mentioned topics in a seminar, solving the corresponding analytical tasks (e.g., mass spectrometry in environmental analysis, food analysis, MALDI-TOF in analysis of polymers, peptide sequencing).
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 26 hours per semester
- Homework for Teaching
- 39 hours per semester
- Preparation for the Course Credit
- 55 hours per semester
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Learning outcomes
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To acquaint students with procedures and applications of mass spectrometry.
Apply theoretical knowledge for the solution of model problems in the field of mass spectrometry
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Prerequisites
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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Assessment methods and criteria
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Oral exam
Student has to prepare and give a talk concerning a selected topic, he has to be able to answer the related questions. During semester, he has to discuss the various topics with other students. During colloquium, student has to be able to give his opinion to any kicked around topic and to interpret mass spectra. Student has to prepare and to give a talk concerning a selected topic, he has to be able to response related questions. During semester, he has to discuss with other students their topics. During colloquium, student has to be able to give his opinion to any kicked around topic and to interpret mass spectra.
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Recommended literature
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Dass Ch.: Fundamentals of contemporary mass spectrometry, John Wiley & Sons, Hoboken 2007..
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de Hoffmann E., Stroobant, V.: Mass Spectrometry. Principles and Applications. John Wiley and sons, Chichester, 2002..
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Doporučená aktuální časopisecká literatura.
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Doporučené aplikace a technická sdělení předních výrobců hmotnostních spektrometrů..
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Feider C.L., Krieger A., DeHoog R.J., Eberlin L.S.: Ambient Ionization Mass Spectrometry: Recent Developments and Applications. Anal. Chem. 91, 4266 (2019)..
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Gross J. H.: Mass Spectrometry, A Textbook, 3. vyd. Springer International Publishing AG, Cham 2017.
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Niessen W.M.A.: Liquid Chromatography-Mass Spectrometry, Marcel Dekker, New York, 1999..
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Sparkman O. D., Penton Z. E., Kitson F. G.: Gas chromatography and mass spectrometry: a practical guide, 2. vyd. Academic Press, Burlington 2011..
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Watson J.T.: Introduction to Mass spectrometry, Lippincott-Raven Publishers, Philadelphia, 1997..
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Wilkins Ch. L., Trimpin S. (ed.): Ion mobility spectrometry-mass spectrometry, Theory and applications, CRC Press, Taylor & Francis Group, Boca Raton 2011..
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