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Lecturer(s)
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Petr Jan, doc. RNDr. Ph.D.
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Course content
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1) Analysis of low-molecular-weight compounds - determination of inorganic and organic ions, applications of microchip electrophoresis. 2) Chiral separations - chiral selectors and their properties, cyclodextrins, optimization, migration order of enantiomers. 3) Analysis of biomacromolecules - separation of proteins, peptides, and nucleic acids, biopharmaceutical analysis. 4) Analysis of nanoparticles and other large objects - specific behavior of nanoparticles, CE-ICP-MS coupling, Taylor dispersion analysis. 5) Physicochemical applications of electromigration techniques - determination of dissociation constants, measurement of electrical conductivity, affinity capillary electrophoresis. 6) On-line preconcentration techniques - principles and applications of preconcentration methods, stacking, sweeping, isotachophoresis, dynamic pH junction. 7) Advanced applications of capillary electrophoresis - glycomics and metabolomics, derivatization, CE-MS. 8) Simulation of electromigration separations - transport equations, system zones, PeakMaster and Simul software. 9) Development and optimization of electromigration methods - strategies, selection of separation systems and detection conditions, troubleshooting. 10) Validation of electromigration methods - practical aspects and validation procedures.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 13 hours per semester
- Homework for Teaching
- 13 hours per semester
- Preparation for the Course Credit
- 34 hours per semester
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Learning outcomes
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The aim of the course is to introduce students to advanced applications of electromigration techniques in analytical chemistry. Emphasis is placed on their use in the analysis of various types of compounds, physicochemical characterization, and the development, optimization, and validation of analytical methods. Students will also become familiar with the possibilities of simulating electromigration separations.
Upon completion of the course, students will be able to select appropriate electromigration techniques for specific analytical problems, interpret the results of capillary electrophoresis analyses, develop and optimize separation methods, evaluate their analytical performance characteristics, and validate analytical methods.
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Prerequisites
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Knowledge of the fundamentals of analytical chemistry, instrumental methods, and theoretical principles of electromigration techniques.
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Assessment methods and criteria
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Oral exam
The colloquium is conducted orally and consists of a discussion of two questions, one focusing on applications of electromigration techniques and the other on the development, optimization, and validation of analytical procedures.
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Recommended literature
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Bob W. J. Pirok, Peter J. Schoenmakers. Analytical Separation Science. 2025.
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Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho. Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations. 2013.
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Colin F. Poole (ed.). Capillary Electromigration Separation Methods. 2018.
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Dale R. Baker. Capillary Electrophoresis. 1995.
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James P. Landers (ed.). Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques. 2008.
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Reinhard Kuhn, Sabrina Hoffstetter-Kuhn. Capillary Electrophoresis: Principles and Practice. 1993.
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Ziad El Rassi (ed.). Advances in Microscale Electro- and Liquid Phase-Based Separation Techniques. 2026.
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