1. Introduction: history and basic principles of solid-phase organic synthesis, its comparison to synthesis in solution. Analytical methods for support-bound and cleaved intermediates: combustion analysis, colorimetric assays, IR spectroscopy, mass spectrometry and NMR spectroscopy. 2. Supports for SPOS: parameters and properties (including advantages and drawbacks) of currently available polymer supports (polystyrene, grafted polystyrene and macroporous polystyrene resins, polyacrylamides, polysaccharides, polyethyleneglycols and miscellaneous supports). 3. Linkers for SPOS: linker types and functions, selection of suitable linker, examples and applicability of the most common linkers (electrophilical and nucleophilical cleavage, photocleavage, reductive and oxidative cleavage, enzymatic cleavage). 4. SPOS of peptides (SPPS): historical overview, linker strategy for the first amino acid immobilization (immobilization via N, C - terminus, side chain, backbone immobilization), coupling methods, preparation of cyclic peptides, protective groups for SPPS. 5. SPOS of oligonucleotides: historical overview, the phosphotriester method, the phosphoramidite and H-phosphonate methods. 6. SPOS of heterocycles: strategy selection for the preparation of representative heterocyclic compounds. 7. Strategies for CSPOS: definition of combinatorial chemistry, definition of diversity, chemical libraries, diversity orientated synthesis, target orientated synthesis; parallel synthesis, split-split method, split-mix method (random and directed) 8. One bead-one one compound (OBOC) method: principle, historical overview, OBOC encoding methods, OBOC screening methods. 9. Resin formulation for directed split-mix method: T-bags, paper discs, resin capsules, wafers, lanterns, Kans. Chemical history of resin formulations: labeling, Rf tags, necklace, spindles and cogs. 10. Equipment and instrumentation for SPOS and CSPOS: manual solid-phase synthesis, integrated semi-automated synthesis, fully automated synthesizers, instruments for sorting and for purification of chemical libraries.
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