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Lecturer(s)
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Hlaváč Jan, prof. RNDr. Ph.D.
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Course content
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1. Fluorescence basis a. Fluorescence principles b. Excitation/emission spektra, anisotropy, Stokes shift, quantum yield c. Instrumentation 2. Fluorophores a. Fluorophores in living systems b. Extrinsic fluorophores for biomolecules labelling c. Quantum dots d. Metal complexes 3. Factors affecting the fluorescence a. Solvent effects b. Other factors affecting the fluorescence 4. Fluorescence quenching a. Mechanism of fluorescence quenching b. Examples and applications of fluorescence quenching 5. Fluorescence energy transfer (FET) a. Principles and conditions of FET b. Transfer effectivity c. Examples and practical applications 6. Fluorescent proteins a. Aminoacids and proteins with fluorescent properties b. Protein structure and fluorescence c. Fluorescent proteins in practical application 7. Fluorescent probes a. Intensiometric and ratiometric probes -comparison, examples b. Multifunctional probes - examples and principles 8. Application of fluorescence in molecular a. Logic gates based on fluorescence b. Application of molecular electronics devices for detection
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook)
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Learning outcomes
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Students will learn the basic principles of fluorescence followed by appropriate chapters aimed to practical applications of phenomena connected with fluorescence. The application will be aimed to bioorganic chemistry and chemical biology. The students should be well informed about application of fluorescence for sensing of the biological processes, labelling of biomolecules, they should know the principles of fluorescent techniques for these purposes and the factors effecting fluorescent detection.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
The students should have good knowledge from the organic chemistry and basic knowledge from spectroscopy. They should be able to evaluate properties of organic compounds and their behavior in appropriate surrounding.
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Recommended literature
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Original papers relevant to the topic.
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Albani, J. R. (2007). Principles and applications of fluorescent spectroscopy. Wiley-Blackwell.
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Lakowicz, J. R. (2006). Principles of fluorescent spectroscopy; 3rd edition. Springer.
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