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Lecturer(s)
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Urban Milan, prof. RNDr. Ph.D.
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Course content
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1. Brief introduction to molecular biology - DNA, RNA, proteins, epigenetics, cell cycle, cell signallization. 2. Physical interactions of molecules in living organisms, interaction of substrate-enzyme, ligand-receptor, drug-target, protein-protein interactions, biomembranes etc. 3. Design of small molecules that influence the interactions; the relationship between binding energy and activity. 4. Design of molecules that mimic nucelic acids, proteins and molecules that have influence on gene expression. 5. Basic laboratory techniques used in molecular medicine - isolation, purification, and characterization of nucleic acids and proteins, gene expression analysis, fluorescent reporter assays. 6. List of the most commonly used fluorescent molecules used in biological experiments. 7. Behavior of xenobiotics in the living organism, adsorption, transport mechanisms, metabolic transformations, excretion. 8. Chemical modifications of small molecules that rationally change their behavior in biological system.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook)
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Learning outcomes
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This lecture is focused on chemical reactions in living organisms and on the possibilities how to influence or interfere, monitor or mimic the biological processes. The main focus will be oriented to compounds usable in diagnostics, compounds with potential therapeutic effect whether they currently are or are not being commercially used. Future trends of this field will be discussed.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
The candidate is expected to conduct scientific discussion in broader context of the whole discipline.
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Recommended literature
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Alberts, B., Wilson, J., & Hunt, T. (2015). Molecular biology of the cell. New York, N.Y: Garland Science.
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Nelson, D.L., Cox M.M. (2017). Lehninger Principles of Biochemistry, 6th edition. Macmillan.
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Slabý, O. et al. (2015). Molekulární medicína. Galén.
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Van Vranken, D.; Weiss, G. (2013). Introduction to Bioorganic Chemistry and Chemical Biology. Garland Science, Taylor a Francis Group, LLC.
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