| Course title | Chemical Biology I |
|---|---|
| Course code | OCH/CB1 |
| Organizational form of instruction | Lecture |
| Level of course | Master |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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1. Chemical Biology - introduction, definition of the field, overlook and history 2. Proposals libraries of chemicals for screening of the biological activities. Necessary infrastructure, high-throughtput screening technology. 3. Affinity chromatography, antibodies, immunoprecipitation 4. Working with chemical and biological databases, data mining software tools for analysing data from the High Capacity Testing 5. Gene reporters, luciferase, luminescent technology mapping cellular pathways, fluorescent proteins and their applications in chemical biology 6. Proteomics, introduction to the methodology; proteom, biomarkers, separation and identification methods. 7. Analysis of the image, high-content analysis, non label technology, machine learning, interpretation in relation to the SAR. 8. siRNA, shRNA, design, optimization, preparation, principles application in search of small molecule targets 9. Enzymatic and biochemical tests, enzyme kinetics 10. In situ click chemistry and HALO proteins, covalent chemical interactions such as interactions analogy antibody - protein. 11. Evaluation of interaction at the level of individual molecules - FRET colocalization AFM, SNOM, SELEX aptamers superresolution, biomolecular interactions - SPR Yeast two hybrid chips. 12. Journal club
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| Learning activities and teaching methods |
| Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming) |
| Learning outcomes |
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This class is focused on basic applications of chemical biology in the drug development. Students will learn to work with the information sources and databases of chemical biology. Key methodical approaches used for the testing of chemical libraries and performing of biological experiments will be presented.
Understanding of the biological processes at molecular level and theoretical background of modern methods used in chemical biology |
| Prerequisites |
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unspecified
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| Assessment methods and criteria |
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Oral exam, Seminar Work
Knowledge of the subject matter (syllabus) and recommended literature. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Bioorganic Chemistry and Chemical Biology (2020) | Category: Chemistry courses | 1 | Recommended year of study:1, Recommended semester: Summer |