| Course title | Fundamentals of Proteomics |
|---|---|
| Course code | KBI/ZPEN |
| Organizational form of instruction | Lecture + Seminar |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 5 |
| Language of instruction | English |
| Status of course | Optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
|---|
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| Course content |
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1. Introdution to proteomics, definition, history, application of proteomics in biological experiments, advantages, limitations, top-down and bottom-up approaches 2. Application of proteomics in biological experiments: developmental proteomics, proteomics of stress response, proteomics of mutants, organelle proteomics, clinical proteomics 3. The workflow of proteomic analysis, overview on proteomic methods 4. Sample preparation ? principles of protein sample handling, homogenization, protein solubilisation, precipitation, fractionation, detergents 5. Two-dimensional electrophoresis ? principle, application, isoelectric focusing with demonstration, polyacrylamide electrophoresis, protein staining, 6. Two-dimensional electrophoresis-gel documentation, software analysis, profiling 7. Gel-free proteomic analyses: principles, peptide separation, 8. Protein dugextion theory, principles, peptide separation 9. Introduction into mass spectrometry: theory, demonstration of mass spectrometer 10. Introduction into the interpretation and evaluation of proteomic data 11. Posttranslational modification of proteins: theory, detection using proteomic methods 12. Protein phosphorylation: principles, importance, phosphoproteomics
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| Learning activities and teaching methods |
| Lecture, Work with Text (with Book, Textbook) |
| Learning outcomes |
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Acquiring basic knowledge in the field of proteomics.
Students will gain an overview of basic concepts in proteomics, the methods used in proteomics, and the applications of proteomics in biotechnology. They will also become familiar with different strategies of proteomic analysis. |
| Prerequisites |
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unspecified
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| Assessment methods and criteria |
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Written exam
Completion and regular presentation of all tasks given by the lecturer. 100% attendance on seminars. Successful completion of a written exam in the form of a test with a minimum score of 65%. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biotechnology and Genetic Engineering (2026) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Summer |