| Course title | Fundamentals of Proteomics |
|---|---|
| Course code | KBI/ZPA |
| Organizational form of instruction | Lecture + Seminar |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 5 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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1. Introdution to proteomics, definition, history, application of proteomics in biological experiments, advantages, limitations, top-down and bottom-up approaches 2. Application of proteomics in biological experiments: developmental proteomics, proteomics of stress response, proteomics of mutants, organelle proteomics, clinical proteomics 3. The workflow of proteomic analysis, overview on proteomic methods 4. Sample preparation ? principles of protein sample handling, homogenization, protein solubilisation, precipitation, fractionation, detergents 5. Two-dimensional electrophoresis ? principle, application, isoelectric focusing with demonstration, polyacrylamide electrophoresis, protein staining, 6. Two-dimensional electrophoresis-gel documentation, software analysis, profiling 7. Gel-free proteomic analyses: principles, peptide separation, 8. Protein dugextion theory, principles, peptide separation 9. Introduction into mass spectrometry: theory, demonstration of mass spectrometer 10. Introduction into the interpretation and evaluation of proteomic data 11. Posttranslational modification of proteins: theory, detection using proteomic methods 12. Protein phosphorylation: principles, importance, phosphoproteomics
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| Learning activities and teaching methods |
Lecture, Work with Text (with Book, Textbook)
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| Learning outcomes |
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Acquiring basic knowledge in proteomics.
Students gain insichts into the fundamental proteomic terminology, methods and applications. They will gain knowledge about diverse proteomic workflows. |
| Prerequisites |
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unspecified
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| Assessment methods and criteria |
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Written exam
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| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biotechnology and Genetic Engineering (2022) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |