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Lecturer(s)
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Medelská Miroslava, Mgr. Ph.D.
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Šamajová Olga, Mgr. Dr.
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Krasylenko Yuliya, Ph.D.
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Hlaváčková Kateřina, Mgr. Ph.D.
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Course content
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Basic terms, history and principles of in vitro techniques. Principles aseptic handling and cultivation of the plant material. Nutrition tissue cultures - the composition of culture media, growth regulators. Physical culture conditions. Basic types of methods in vitro cultures of plant - plant , embryo , organ , mesh , cellular , protoplast. Micropropagation. Totipotency of plant cells. Morphogenesis and its regulation. Organogenesis. Somatic embryogenesis. Haploid technology - androgenesis, gynogeneze. Regeneration of plants. Somaclonal variability. Preservation of the gene pool in the form of in vitro cultures. The use of tissue cultures in biotechnology practice. Transgenesis. Laboratory exercises will focus on practical mastering the principles of aseptic work with plant material, preparation of culture media, establishment of tissue culture and cultivation and propagation of plant material in vitro. The role of students will separate the establishment of tissue culture for somatic embryogenesis and its subcultures to obtain regenerated plants and subsequent transfer to ex vitro conditions. Different stages (callogenesis, embryogenesis and regeneration of plants) will be documented visually and statistically evaluated. Part of the laboratory exercises will be a practical demonstration of the use of tissue cultures in biotechnology practice - the transformation of explants with Agrobacterium tumefaciens and subsequent induction of somatic embryogenesis.
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Learning activities and teaching methods
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Lecture, Observation, Laboratory Work
- Homework for Teaching
- 2 hours per semester
- Attendace
- 52 hours per semester
- Preparation for the Course Credit
- 4 hours per semester
- Preparation for the Exam
- 40 hours per semester
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Learning outcomes
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The aim of Plant tissue culteres subject is to gain knowledge about the cultivation of plant material in vitro and on the use of tissue cultures in biotechnology practice. Practical working knowledge of the principles of aseptic manipulations with plant material, preparation of tissue culture and plant cultivation in vitro conditions.
After completing the course the student should be familiar with tissue culture methods and their applicability in biotechnology practice. It should also be able to handle independently the work with the plant material in sterile conditions and preparation of tissue cultures.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written exam
Attendance at lectures and seminars, final test, protocols.
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Recommended literature
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Loyola-Vargas V.M., Vázquez-Flota F. (2006). Plant cell culture protocols, 2-nd edition. Humana Press, Totowa.
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Smith R.H. (2000). Plant tissue culture. Techniques and experiments, 2-nd edition. Academic Press, San Diego.
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Šamaj J., Bobák M., Ovečka M., Blehová A., Preťová A. (1997). Structural features of plant morphogenesis in vitro. Veda, Bratislava.
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Trigiano R.N, Gray D.J. (2011). Plant tissue culture, development and biotechnology. CRC Press, Boca Raton.
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Wang K. (2006). Agrobacterium protocols, 2-nd edition. Humana Press, Totowa.
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