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Lecturer(s)
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Course content
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1) History of plant protection and the importance of molecular plant pathology. Basic glossary of plant pathology. 2) The genetic basis of plant-pathogen interaction. Virulence and avirulence , "gene-for-gene" theory, oligogenic resistance and polygenic resistance. 3) R genes, methods used for the identification and isolation of R genes, molecular structure and function of R genes, "slow rusting" R genes, R gene Mlo. 4) Molecular bases for virulence and avirulence of plant pathogens. Effectors: definition, function and delivery into host tissues. Examples. 5) Molecular co-evolution of R genes and effector genes, diversifying and stabilizing selection. 6) Classification and characterization of molecular interactions between effectors and R proteins. Prediction of R gene stability. Examples. 7) Functional genomics of plant pathogen effectors (effectoromics) as a tool for co-identification of R and Avr genes. In planta identification of R-AVR interactions. 8) Current status of effectoromics in most important pathogens. Utilization of effectoromics for plant pathogen resistance breeding. 9) Plant resistance to pathogens controlled by QTL. Model pathosystem Puccinia hordei-Hordeum vulgare. Methods used for the identification and evaluation of QTLs for partial resistance. 10) Molecular basis underlying QTLs for plant partial resistance to pathogens. "Microbe Associated Molecular Patterns" (MAMP). 11) The role of mitogen-activated protein kinases in plant host responses to pathogen attack. 12) Summary, current models of molecular interactions between host plant and invading pathogen. The practical seminar is focused on in planta identification of R-AVR interactions following transient co-transformation of Nicotina benthamiana by candidate R and Avr genes. Seminar is taught in two individual time blocks.
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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Student will understand to the major principles of molecular battle between host plant and invading pathogen.
Student will acquire basic knowledge in molecular genetics and genomics aspects of plant pathogen interaction.
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Prerequisites
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Completion of the course CRH / ZGI Fundaments of genetic engineering.
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Assessment methods and criteria
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Written exam
The prerequisite for the course is a written examination (test 1 hour 12 questions) with a minimum of 15 points out of 24 possible points. Credit is awarded for completing the exercise.
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Recommended literature
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Agrios, N. (2005). Plant Pathology, Fifth Edition. Academic Press.
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Dickinson, M. (2003). Molecular plant pathology. School of Biosciences, University of Nottingham, BIOS Scientific Publishers.
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Kůdela, V. (Ed.). (1989). Obecná fytopatologie. Academia, Praha.
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Martin, F., & Kamoun, S. (2012). Effectors in plant-microbe interactions. Chichester: Wiley-Blackwell.
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