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Lecturer(s)
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Luptovčiak Ivan, Mgr. Ph.D.
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Dvořák Petr, Mgr. Ph.D.
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Course content
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-preparation of bacterial material and tools including nutrient media, induction of expression -lysis of cells, extraction and purification of the expressed protein -methods for determining protein concentration and also methods for determining superoxide dismutase (SOD) enzyme activity -processing of experimental data and image material -evaluation of experiments and preparation of protocols (word and/or pdf document)
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work
- Semestral Work
- 10 hours per semester
- Attendace
- 50 hours per semester
- Homework for Teaching
- 5 hours per semester
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Learning outcomes
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Familiarize yourself with microorganism cultivation techniques, techniques used in down-stream processing, as well as selected methods for recombinant protein analyses. Students work in groups under the guidance of professional staff..
After completing this course, the student will gain theoretical and practical experience in the areas of: cultivation of microorganisms, induced protein expression, extraction and purification of recombinant proteins, methods for determining protein concentration, as well as methods for determining SOD enzyme activity (both gel-free and gel-based procedures).
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Prerequisites
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Master's studies in biologically or chemically oriented fields at the Faculty of Science, UP. A basic knowledge of microorganism handling techniques is recommended.
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Assessment methods and criteria
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Student performance, Seminar Work
100% participation, completion of experimental tasks, preparation and submission of structured exercise protocols no later than two weeks after the end of the course.
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Recommended literature
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