Practical application of advanced methods of light microscopy, DIC in the study of live unstained objects, videomicroscopy and recording of dynamic processes using different types of cameras. Fluorescence microscopy, documentation and software quality adjustment of noisy images using deconvolution. Confocal laser scanning microscopy and its practical application in the study of plant cells. Advanced methods of studying fluorescently labeled epitopes in living plant cells, co-localization, spectral analysis, FRAP, FLIP, FRET, physiological measurements. Scanning of dynamic cellular processes in real time using confocal microscopy with a spinning disk, practical analysis and evaluation of dynamic processes taking place in living plant cells. 1. Light microscopy, scanning of fast processes in living plant cells by methods of contrast modulation and videomicroscopy. 2. Fluorescence microscopy, deconvolution and its application in the scanning of fluorescently labelled objects. 3. Confocal laser scanning microscopy, multichannel scanning, time-lapse imaging and 3D reconstruction. 4. Confocal laser scanning microscopy, determination of mobility and stability of fluorescently labelled epitopes by methods of co-localization, FRAP and FLIP. 5. Confocal laser scanning microscopy, study of epitope interactions using FRET. 6. Confocal laser scanning microscopy, spectral characterization of fluorochromes, separation of emission spectra. 7. Confocal laser scanning microscopy, physiological studies by determining the intracellular concentration and distribution of molecules. 8. Confocal microscopy with a spinning disc, principle of the method, sample preparation for live cell imaging and scanning of fast cellular processes. 9. Confocal microscopy with the spinning disc, scanning of the structure of fluorescently labelled cytoskeleton in plant cells. 10. Confocal microscopy with the spinning disc, scanning of the dynamic motion of fluorescently labelled organelles in plant cells. 11. Confocal microscopy with a spinning disk, data evaluation of the structure and mobility of cytoskeletal and membrane cell structures. 12. Confocal microscopy with a spinning disc, multi-channel microscopy of fast processes in living plant cells.
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Price RL, Jerome WG. (2010). Basic confocal microscopy. Springer, Heidelberg.
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