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Lecturer(s)
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Šebela Marek, prof. Mgr. Dr.
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Petřivalský Marek, prof. Mgr. Dr.
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Course content
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Principles and functions of redox signalling, the relevance of redox signalling within signalling networks based on ligand-receptor interactions, evolutionary aspects of redox signalling in prokaryotes and eukaryotes; importance of free radicals in living organisms. Reactive forms of oxygen (ROS): pathways of enzyme- and non-enzyme ROS production, ROS reactivity and their catabolism, antioxidant enzymes and low-molecular antioxidants. Reactive forms of nitrogen (RNS): pathways of enzyme- and non-enzyme RNS production, RNS reactivity and their catabolism. Reactive forms of sulphur (RSS): pathways of enzyme- and non-enzyme RSS production, RSS reactivity and their catabolism. Regulation of biomolecules structures and activity by redox modification, redox modification of protein aminoacids, oxidative modification of cysteine, oxidative and nitration modifications of nucleic acids and free nucleotides, mechanisms of reversibility. Redox homeostasis and pathophysiological consequence of its disturbance, importance of redox signalling in regulation of metabolic processes in cells and their responses to external stimuli and stress conditions. Oxidative, nitrosative and nitro-oxidative stress. Specific feature od redox signalling in microbial, plant and animal cells, specific redox signalling pathways of cell compartments and eukaryotic organelles. Redox signalling in interaction of eukaryotic organisms and microbial pathogens. Current methods in studies of redox signalling, methods of analysis of ROS, RNS and RSS, methods of analysis of protein post-translational modifications.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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The subject will introduce to the field of redox signalling in context of intracellular signalling pathways and their regulation; roles of reactive species of oxygen, nitrogen and sulphur in redox signalling; covalent modifications of signalling, effector and regulatory proteins. The subject will provide with an overview of the importance of specific types of redox signalling in diverse types of organisms and experimental methods used in studies of redox signalling pathways.
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Prerequisites
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Successful graduated studium of Bachelor of Science and Magister of Science in Biochemistry.
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Assessment methods and criteria
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Oral exam
Oral examination - successfuly answered three questions of general knowledge.
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Recommended literature
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Bak DW, Weerapana E. Cysteine-mediated redox signalling in the mitochondria. 2015 Mol. BioSyst. 11: 678-697.
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Brewer TF, Garcia FJ, Onak CS et al. Chemical Approaches to Discovery and Study of Sources and Targets of Hydrogen Peroxide Redox Signaling Through NADPH Oxidase Proteins. 2015 Annu. Rev. Biochem. 84:765-790.
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Holmström KM, Finkel T. Cellular mechanisms and physiological consequences of redox-dependent signalling. 2014 Nature Rev. Mol. Cell Biol. 15: 411-421.
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Rudolph TK, Freeman B. Transduction of Redox Signaling by Electrophile- 2009 Protein Reactions. Sci. Signal. 2, re7.
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Schieber M, Chandel NS. ROS Function in Redox Signaling and Oxidative Stress. 2014 Curr Biol. 24: R453-R462..
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Zhang J, Wang X, Vikash V et al. ROS and ROS-Mediated Cellular Signaling. 2016 Oxid. Med. Cell. Longev. Article ID 4350965.
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