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Lecturer(s)
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Janků Martina, Mgr. Ph.D.
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Strádalová Zuzana, Mgr.
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Slivková Jana, Mgr.
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Tölgová Linda, Mgr.
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Hofericová Gabriela, Mgr.
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Hrtánková Eva, Mgr.
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Rašková Michaela, Mgr.
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Jedelská Tereza, Mgr. Ph.D.
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Jemelková Jana, Mgr. Ph.D.
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Jahnová Jana, Mgr.
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Course content
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Basic skills in biochemistry laboratory: weights, pipetting technique, dilution series, preparation of buffers. Basic methods in biochemistry laboratory: homogenization of biological material, extraction and precipitation, filtration and centrifugation, membrane separation (dialysis and ultrafiltration), spectrophotometry (calibration curve, concentration measurements), chromatography (gel permeation chromatography, TLC), electromigration methods (agarose electrophoresis), distillations, sublimation, melting point determination, titration, conductometry, microscopic methods and analyses.
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Learning activities and teaching methods
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Demonstration, Laboratory Work
- Homework for Teaching
- 50 hours per semester
- Attendace
- 65 hours per semester
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Learning outcomes
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Basic methodological approaches for biochemistry
Apply and practice fundamental laboratory operations.
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Prerequisites
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Student of the Biochemistry or BGI study program
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Assessment methods and criteria
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Written exam, Student performance
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Recommended literature
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Anzenbacher P., Kovář J. (1986). Metody chemického výzkumu pro biochemiky. MŠ ČSR Praha.
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Galuszka P., Luhová L. Laboratorní technika pro biochemiky, UP Olomouc, 2003.
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Peč P. a kol. Laboratorní cvičení z biochemie, UP Olomouc, 2000.
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Pingoud A., Urbanke C., Hoggett J., Jeltsch A. (2002). Biochemical Methods: A Concise Guide for Students and Researchers.. Wiley-VCH.
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